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MABN812 Anti-VPS4A Antibody, clone 14B1.1

Detect VPS4A using this Anti-VPS4A Antibody, clone 14B1.1 validated for use in Western Blotting and Immunohistochemistry (Paraffin).

Anti-VPS4A Antibody, clone 14B1.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: VPS4A, VPS4 UniProt Number: Q9UN37
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MABN812
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN812
      DescriptionAnti-VPS4A Antibody, clone 14B1.1
      Alternate Names
      • Vacuolar protein sorting-associated protein 4A
      • Protein SKD2
      • VPS4-1
      • hVPS4
      • VPS4A
      Background InformationVacuolar protein sorting-associated protein 4A (EC 3.6.4.6; UniProt Q9UN37; also known as hVPS4, Protein SKD2, SKD1-homolog, Vacuolar protein sorting factor 4A, Vacuolar sorting protein 4, VPS4A) is encoded by the VPS4A gene (also known as SKD1A, VPS4, VPS4-1) (Gene ID 27183) in human. Vps4 is a member of the ATPases associated with various cellular activities. The ESCRT (endosomal sorting complexes required for transport) machinery consists of five complexes (SCRT-0, ESCRT-I, ESCRT-II, ESCRT-III, and Vps4) that mediate multiple cellular membrane remodeling events, ranging from multivesicular bodies and exosomes formation to the final membrane abscission stage of cytokinesis, all of which converge in the scission of the narrow membrane neck that connects bud to parent membrane or daughter cell to daughter cell. Scission depends on the polymerization of soluble ESCRT-III monomers or dimers into tightly membrane-bound filaments. Vps4 is essential for ESCRT function in cells and it mediates the disassembly of ESCRT-III filaments to ensure a constant replenishing of the pool of soluble ESCRT-III monomers. The ESCRTs are also exploited by HIV-1 and many other enveloped viruses for their escape from the host cells. Vps4A is reported to facilitate the secretion of oncogenic miRNAs in exosomes and downregulation of Vps4A in hepatocellular carcinoma (HCC) is associated with tumor progression and metastasis.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect VPS4A using this Anti-VPS4A Antibody, clone 14B1.1 validated for use in Western Blotting and Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected VPS4A in 200 µg of MCF-7 cell lysate.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected VPS4A in rat cerebral cortex tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human VPS4A sequence at the N-terminus.
      EpitopeNear N-terminus
      Clone14B1.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VPS4A
      • VPS4
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~50 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected VPS4A in 200 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN812 04055977173499