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ABE409 Anti-UTX Antibody

Anti-UTX, Cat. No. ABE409, is a rabbit polyclonal antibody that detects Histone demethylase UTX and is tested for use in Immunocytochemistry, Peptide Inhibition Assay, and Western Blotting.

Anti-UTX Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: KDM6A, UTX UniProt Number: O15550
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ABE409
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE409
      DescriptionAnti-UTX Antibody
      Alternate Names
      • Lysine-specific demethylase 6A
      • Histone demethylase UTX
      • Ubiquitously-transcribed TPR protein on the X chromosome
      • Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein
      Background InformationLysine-specific demethylase 6A (UniProt: O15550; also known as EC:1.14.11.68, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein, [histone H3]-trimethyl-L-lysine(27) demethylase 6A, KDM6A, UTX) is encoded by the KDM6A (also known as UTX) gene (Gene ID: 7403) in human. UTX is a histone demethylase that specifically demethylates lysine 27 of histone H3 and plays a central role in histone code. It is shown to demethylate trimethylated and dimethylated, but not monomethylated H3 lysine 27. It mediates muscle regeneration through its demethylase activity, while it regulates mesoderm differentiation of embryonic stem cells and mammary gland development independent of its demethylase activity. It also plays a role in regulating brown adipocyte thermogenic program. Its levels are shown to be up-regulated during brown adipocyte differentiation and by cold exposure in both brown and white adipose tissue in mice, indicating its potential role in thermogenesis. Mutations in KDM6A gene are a causative factor in Kabuki syndrome that is characterized by congenital mental retardation, postnatal dwarfism, large prominent earlobes, a cleft or high-arched palate, scoliosis, and other abnormalities. (Ref.: Zha, L et al. (2015). J. Biol. Chem. 290, 25151-25163; Lederer, D et al. (2012). Am. J. Hum. Genet. 90, 119-124).
      Product Information
      FormatAffinity Purified
      Control
      • HeLa nuclear extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-UTX, Cat. No. ABE409, is a rabbit polyclonal antibody that detects Histone demethylase UTX and is tested for use in Immunocytochemistry, Peptide Inhibition Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Peptide Inhibition Assay
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected UTX in HeLa, A431, and NIH3T3 cells.

      Peptide Inhibition Assay: Target band detection in nuclear extract of HeLa cells was prevented by preblocking of a representative lot with the recombinant UTX protein.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 82 amino acids from the N-terminal half of human Histone demethylase UTX.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Histone demethylase UTX. It targets an epitope within the N-terminal half.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Bovine, Canine, Chimpanzee based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KDM6A
      • UTX
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~155 kDa observed; 154.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in nuclear extract of HeLa cells.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UTX in nuclear extract of HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      ABE409 04053252913976