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Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
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-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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ABT256
Sigma-AldrichAnti-Telo-Collagen Type I, A1/COL1A1
Anti-Telo-Collagen Type I Antibody, A1/COL1A1 is an antibody against Telo-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.
More>>Anti-Telo-Collagen Type I Antibody, A1/COL1A1 is an antibody against Telo-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry. Less<<
Anti-Telo-Collagen Type I, A1/COL1A1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Collagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human. Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain. Alpha-1 type I collagen is initially produced as an 1464-amino acid prepro-form with a signal peptide sequence (a.a. 1-22) and two propeptide sequences (a.a. 23-161 and a.a. 1219 –1464), the removal of which yields the mature alpha-1(I) chain. The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a. 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a. 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a. 1193-1218; numbering based on the prepro-form). Collagen can be extracted from tissue via either enzymatic or non-enzymatic means. Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin. On the other hand, collagen preparations obtained with non-enzymatic means (e.g. by acid extraction) have the intact telopeptides at both ends.
References
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Anti-Telo-Collagen Type I Antibody, A1/COL1A1 is an antibody against Telo-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.
Key Applications
Western Blotting
Flow Cytometry
Immunohistochemistry
Application Notes
Western Blotting Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung and skin tissue lysate. Flow Cytometry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue. Immunohistochemistry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue.
Biological Information
Immunogen
Linear peptide corresponding to sequence derived from human Collagen Type I, A1/COL1A1 C-terminal telopeptide region.
Epitope
C-terminal telopeptide region
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
Cat. No. ABT256 Anti-Telo-Collagen Type I, A1/COL1A1 was raised against a sequence derived from the C-terminal telopeptide (not the N-terminal telopeptide) region. This polyclonal antibody will detect prepro-, pro-, and mature collagen alpha-1(I) chain, but not atelocollagen prepared with proteolytic enzymes due to the absence of the C-terminal telopeptide sequence.
Species Reactivity
Human
Mouse
Sheep
Species Reactivity Note
Human, Mouse, Sheep. Predicted to react with a broad range of species based on high sequence homology, including mammalian, avian, and amphibian species.
Evaluated by Western Blotting in mouse liver tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Telo-Collagen Type I, A1/COL1A1 in 1 µg of mouse liver tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
