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654250 Anti-TNF-α Rabbit pAb

This Anti-TNF-α Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Neutralization Studies, Paraffin Sections, Radioimmunoassay for the detection of TNF-α.

Anti-TNF-α Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tumor Necrosis Factor-α

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654250
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Pricing & Availability

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654250-1MG
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      Description
      OverviewRecognizes recombinant and monocyte-derived TNF-α. Exhibits some cross-reactivity with lymphotoxin (TNF-β) and mouse TNF-α in L929 cells.
      Catalogue Number654250
      Brand Family Calbiochem®
      SynonymsAnti-Tumor Necrosis Factor-α
      References
      Product Information
      Unit of Definition1 EU corresponds to 0.1 ng endotoxin (EC-6/USP Standard)
      FormLiquid
      FormulationIn PBS.
      Positive controlL929 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Neutralization Studies
      Paraffin Sections
      Radioimmunoassay
      Application NotesELISA (see comments)
      Frozen Sections (see comments)
      Immunoblotting (see comments)
      Neutralization Studies (see comments)
      Paraffin Sections (see comments)
      Radioimmunoassay (see comments)
      Application CommentsReacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenrecombinant, human TNF-α
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsEndotoxins: ≤1 EU/mg
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      654250-1MG 04055977184617

      Documentation

      Anti-TNF-α Rabbit pAb MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TNF-α Rabbit pAb Certificates of Analysis

      TitleLot Number
      654250
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2009 JSW
      SynonymsAnti-Tumor Necrosis Factor-α
      ApplicationELISA (see comments)
      Frozen Sections (see comments)
      Immunoblotting (see comments)
      Neutralization Studies (see comments)
      Paraffin Sections (see comments)
      Radioimmunoassay (see comments)
      DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes recombinant and monocyte-derived TNF-α.
      BackgroundHuman Tumor Necrosis Factor α (TNF-α, cachectin) is a cytokine, produced by variety of cell types, that is involved in mediation of the inflammatory response, resistance to infection, and resistance to tumor growth. Human and mouse TNF-α are approximately 79% homologous at the amino acid level and exhibit cross-species reactivity. This antibody recognizes both recombinant and natural human TNF-α and exhibits some (~30%) cross-reactivity with mouse TNF-α. It exhibits only minor (<1%) cross-reactivity with human TNF-β (lymphotoxin).
      HostRabbit
      Immunogen speciesHuman
      Immunogenrecombinant, human TNF-α
      IsotypeIgG
      Specieshuman, mouse (weakly)
      Positive controlL929 cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      ContaminantsEndotoxins: ≤1 EU/mg
      Unit definition1 EU corresponds to 0.1 ng endotoxin (EC-6/USP Standard)
      PreservativeNone
      CommentsReacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling