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MABT884 Anti-TACE Antibody, clone D1(A12)

Detect TACE/ADAM17 using this human monoclonal Anti-TACE, clone D1(A12), Cat. No. MABT884, validated for use in Flow Cytometry and Activity/Function Inhibition.

Anti-TACE Antibody, clone D1(A12) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: ADAM17, CSVP, TACE UniProt Number: P78536
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MABT884
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HINHIB, FCHuman RecombinantPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT884
      DescriptionAnti-TACE Antibody, clone D1(A12)
      Alternate Names
      • Disintegrin and metalloproteinase domain-containing protein 17
      • ADAM 17
      • CD156b
      • Snake venom-like protease
      • TNF-alpha convertase
      • TNF-alpha-converting enzyme
      Background InformationDisintegrin and metalloproteinase domain-containing protein 17 (UniProt: P78536; also known as EC:3.4.24.86, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, TACE, CD156b) is encoded by the ADAM17 (also known as CSVP, TACE) gene (Gene ID: 6868) in human. TACE is a membrane-bound metalloprotease responsible for solubilizing many pathologically significant membrane substrates. Mature ADAM-family ectodomains contain a globular metalloprotease catalytic domain, a disulfide-dependent disintegrin cysteine rich (Dis-Cys) domain, and, in some cases, an epidermal growth factor (EGF)-like domain. ADAM17 activity is regulated by multiple post-translational modifications. It has three phosphorylation sites on its C-terminal cytoplasmic domain (T735, S791, and (S819). ERK1/2-mediated T735 phosphorylation is reported to regulate ADAM17 maturation and surface expression. TACE displays a narrow endopeptidase specificity. It cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form and is also responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Clone D1(A12) exhibits high affinity (Kd = 0.46 nM) for the TACE ectodomain and is the most selectively potent cell-surface ADAM inhibitor. It can independently bind both the complete TACE ectodomain and the isolated catalytic domain (Kd = 5.21 nM). It is shown to be about 5-fold more potent than N-TIMP3 in inhibiting cell surface TACE. Mutations in ADAM17 gene are known to cause inflammatory skin and bowel disease, neonatal, 1 (NISBD1) that is characterized by inflammatory features with neonatal onset, involving the skin, hair, and gut.
      References
      Product Information
      FormatPurified
      PresentationPurified recombinant human IgG1 in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect TACE/ADAM17 using this human monoclonal Anti-TACE, clone D1(A12), Cat. No. MABT884, validated for use in Flow Cytometry and Activity/Function Inhibition.
      Key Applications
      • Inhibits Activity/Function
      • Flow Cytometry
      Application NotesInhibits Activity/Function: 6 µg/mL from a representative lot inhibited TACE-mediated CD16 shedding from the surface of stimulated primary human NK cells (Courtesy of Zachary Davis, Ph.D., University of Minnesota, USA).

      Inhibits Activity/Function: Representative lots inhibited TACE-mediated substrates shedding from the surface of human cells both in cultures and in mice in vivo (Issuree, P.D., et al. (2013). J. Clin. Invest. 123(2):928-932; Richards, F.M., et al. (2012). PLoS One. 7(7):e40597; Tape, C.J., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(14):5578-5583).
      Biological Information
      ImmunogenSee Specificity section.
      Epitopeextracellular domain
      CloneD1(A12)
      ConcentrationPlease refer to lot specific datasheet.
      HostHuman Recombinant
      SpecificityCat. No. MABT884 is a recombinant human IgG1 that contains a heavy chain variable (VH) region with affinity towards human TACE noncatalytic Dis-Cys domain and a light chain variable (VL) region that binds human TACE catalytic domain (Tape, C.J., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(14):5578-5583). The VH region of D1(A12) is derived from the scFv named D1 isolated from a phage library generated using pooled lymphocytes from 43 donors (Schofield, D.J., et al. (2007). Genome Biol. 8(11):R254). The D1 VH cDNA was subsequently cloned into a naïve human VL phage-display library for identification and isolation of the hybrid D1(A12) scFv with affinity toward both Dis-Cys domain and the catalytic domain. The D1 VH and A12 VL cDNAs were then cloned into a pBudCE4.1 human IgG1 expression vector for generating the recombinant antibody in HEK293 cells (Tape, C.J., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(14):5578-5583).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman recombinant. Not Mouse (Kwok, H.F., et al. (2014). Protein Eng. Des. Sel. 27(6):179-190.)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ADAM17
      • CSVP
      • TACE
      UniProt Number
      Molecular Weight93.02/91.12/68.22 kDa (prepro-/pro-/mature form) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HeLa cells.

      Flow Cytometry Analysis: 0.2 µg (2 µg/mL) of this antibody detected TACE expression on the surface of one million HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT884 04054839168659

      Documentation

      Anti-TACE Antibody, clone D1(A12) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TACE Antibody, clone D1(A12) Certificates of Analysis

      TitleLot Number
      Anti-TACE, clone D1(A12) - 3026858 3026858
      Anti-TACE, clone D1(A12) - 3808649 3808649
      Anti-TACE, clone D1(A12) - 3833688 3833688
      Anti-TACE, clone D1(A12) - 3861912 3861912
      Anti-TACE, clone D1(A12) - 3872426 3872426
      Anti-TACE, clone D1(A12) - 3906473 3906473
      Anti-TACE, clone D1(A12) - 4003457 4003457
      Anti-TACE, clone D1(A12) - 4059298 4059298
      Anti-TACE, clone D1(A12) -Q2814939 Q2814939
      Anti-TACE, clone D1(A12) Monoclonal Antibody 3128144
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