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04-1572-I Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

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04-1572-I
100 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MChIP, WB, ELISARCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-1572-I
      Replaces04-1572
      DescriptionAnti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
      Alternate Names
      • DNA-directed RNA polymerase II subunit RPB1
      • RNA polymerase II subunit B1
      • DNA-directed RNA polymerase II subunit A
      • DNA-directed RNA polymerase III largest subunit
      • RNA-directed RNA polymerase II subunit RPB1
      Background InformationRPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at 'Ser-7' of the heptapepdtide repeat. This antibody recognizes the 'Ser-5' CTD residue of RPB1.
      Product Information
      FormatCulture Supernatant
      Control
      • Untreated and lambda phosphotase treated NIH/3T3 cell lysates
      PresentationRat monoclonal IgG2aκ with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 is a highly specific rat monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting & ELISA.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      • ELISA
      Application NotesChromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).

      ELISA Analysis: A representative lot was used by an independent laboratory to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminal domain (CTD).
      EpitopePhosphorylated Ser5 at the C-terminus domain (CTD).
      Clone3E8
      HostRat
      SpecificityThis antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminus domain (CTD).
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Human based upon immunogen design.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • POLR2A
      • POLR2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~220 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and lambda phosphotase treated NIH/3T3 cell lysates.

      Western Blot Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-5) in 10 µg of untreated and lambda phosphotase treated NIH/3T3 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      04-1572-I 04053252880230