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MABS1680 Anti-PTEN-alpha Antibody, clone 3A4.1

Detect PTEN-alpha using this mouse monoclonal Anti-PTEN-alpha, clone 3A4.1 Antibody, Cat. No. MABS1680, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-PTEN-alpha Antibody, clone 3A4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: PTEN, 10q23del, BRRS, BZS, CWS1, GLM2, MMAC1, MHAM, TEP1 UniProt Number: P60484-2
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MABS1680
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1680
      DescriptionAnti-PTEN-alpha Antibody, clone 3A4.1
      Alternate Names
      • Phosphatidylinositol 3
      • 4
      • 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN-alpha
      • Mitochondrial PTENalpha
      • Phosphatase and tensin-like protein-alpha
      • Mutated in multiple advanced cancers 1-alpha
      • Mitochondrial phosphatase and tensin protein alpha
      • MMAC1 phosphatase and tensin homolog deleted on chromosome 10-alpha
      Background InformationPhosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (EC 3.1.3.16, EC 3.1.3.48, EC 3.1.3.67; UniProt P60484; also known as Mitochondrial PTENalpha, Phosphatase and tensin-like protein, Mutated in multiple advanced cancers 1, Mitochondrial phosphatase and tensin protein alpha, phosphatase and tensin homolog deleted on chromosome 10) is encoded by the PTEN (also known as 10q23del, BRRS, BZS, CWS1, GLM2, MMAC1, MHAM, TEP1) gene (Gene ID 5728) in human. PTEN is a tumor suppressor that catalyzes the removal of phosphate from phosphoserine, phosphothreonine, and phosphotyrosine residues on substrate proteins, as well as the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate. PTEN plays a major role in controlling cell growth and proliferation, and PTEN gene mutains are found in a large number of cancers at high frequency. PTEN activity, stability, localization, and conformation are controlled through complex regulatory mechanisms, including the reversible phosphorylations of several Ser/Thr (S/T) residues in its unfolded C-terminal tail region (PTEN-Ctail, residues 351 403). Alternative translation start site 519 base pairs upstream of the ATG initiation sequence results in the translational variant of PTEN-alpha (PTEN-Long), including additional 173 N-terminal amino acids to the canonical 403-a.a. PTEN sequence. PTEN-Long is a membrane-permeable phosphatase secreted by PTEN-Long producing cells and can enter other cells as an exogenous PI3K signaling-inhibitory agent. PTEN-Long is reported to induce tumor cell death in vitro and in vivo.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationDetect PTEN-alpha using this mouse monoclonal Anti-PTEN-alpha, clone 3A4.1 Antibody, Cat. No. MABS1680, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PTEN-alpha in human cerebellum, pancreas, and small intestine tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human PTEN-alpha N-terminal fragment.
      Clone3A4.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3A4.1 targets an N-terminal region specific to the spliced isoform alpha (PTEN-Long; P60484-2) and not present in spliced isoform 1 (a.k.a. 55 kDa isoform) or isoform 3.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PTEN
      • 10q23del
      • BRRS
      • BZS
      • CWS1
      • GLM2
      • MMAC1
      • MHAM
      • TEP1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~78 kDa observed. 64.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected PTEN-alpha in 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1680 04054839049644

      Documentation

      Anti-PTEN-alpha Antibody, clone 3A4.1 MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PTEN-alpha Antibody, clone 3A4.1 Certificates of Analysis

      TitleLot Number
      Anti-PTEN-alpha, clone 3A4.1 - 3172414 3172414
      Anti-PTEN-alpha, clone 3A4.1 - 3291379 3291379
      Anti-PTEN-alpha, clone 3A4.1 - 3321799 3321799
      Anti-PTEN-alpha, clone 3A4.1 - 3469434 3469434
      Anti-PTEN-alpha, clone 3A4.1 - 3596227 3596227
      Anti-PTEN-alpha, clone 3A4.1 - 4025989 4025989
      Anti-PTEN-alpha, clone 3A4.1 -Q2735573 Q2735573
      Anti-PTEN-alpha, clone 3A4.1 Monoclonal Antibody 3083566