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OP74 Anti-PKC (Ab-2) Mouse mAb (MC5)

This Anti-PKC (Ab-2) Mouse mAb (MC5) is validated for use in Blocking Studies, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of PKC (Ab-2).

Anti-PKC (Ab-2) Mouse mAb (MC5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Protein Kinase C

OP74
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      B, H, M, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~79-80 kDa protein kinase C α/β1/β2 isoforms in NIH3T3 cells and brain tissue.
      Catalogue NumberOP74
      Brand Family Calbiochem®
      SynonymsAnti-Protein Kinase C
      References
      ReferencesXiao, H., et al. 1994. J. Neurosurg. 81, 734.
      Jin, L., et al. 1993. Am. J. Pathol. 142, 569.
      Nishikawa, M., and Shirakawa, S., 1992. Leuk. Lymphoma 8, 201.
      Wetsel, W.C., et al. 1992. J. Cell. Biol. 117, 121.
      Clark, E.A., et al. 1991. Lab. Invest. 64, 35.
      Hirabayashi, N., et al. 1990. Mol. Carcinog. 3, 171.
      Nishizuka, Y., 1989. Cancer 63, 1892.
      Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Positive controlNIH3T3 cells or brain tissue
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesImmunoprecipitation Yong, S., et al. 1988. Eur. J. Biochem. 173, 247. Original Clone Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Neutralization Studies
      Paraffin Sections
      Application NotesBlocking Studies (5 µg/ml, see comments)
      Immunoblotting (1 µg/ml)
      Immunoprecipitation (see application references)
      Paraffin Sections (5 µg/ml, no pre-treatment required)
      Application CommentsMay be used to block the down regulation of PKC activity. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified bovine brain PKC
      ImmunogenBovine
      Epitopewithin the hinge region of PKC
      CloneMC5
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP74 0

      Documentation

      Anti-PKC (Ab-2) Mouse mAb (MC5) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PKC (Ab-2) Mouse mAb (MC5) Certificates of Analysis

      TitleLot Number
      OP74

      References

      Reference overview
      Xiao, H., et al. 1994. J. Neurosurg. 81, 734.
      Jin, L., et al. 1993. Am. J. Pathol. 142, 569.
      Nishikawa, M., and Shirakawa, S., 1992. Leuk. Lymphoma 8, 201.
      Wetsel, W.C., et al. 1992. J. Cell. Biol. 117, 121.
      Clark, E.A., et al. 1991. Lab. Invest. 64, 35.
      Hirabayashi, N., et al. 1990. Mol. Carcinog. 3, 171.
      Nishizuka, Y., 1989. Cancer 63, 1892.
      Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-October-2007 RFH
      SynonymsAnti-Protein Kinase C
      ApplicationBlocking Studies (5 µg/ml, see comments)
      Immunoblotting (1 µg/ml)
      Immunoprecipitation (see application references)
      Paraffin Sections (5 µg/ml, no pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X-63 mouse myeloma cells (see application references). Recognizes ~79-80 kDa α, β1, and β2 isoforms of protein kinase C.
      BackgroundThe protein kinase C (PKC) family of protein consists of 12 known isozyme members which are classified as Ca++/phosphatidylserine-dependent, serine/threonine kinases. In addition to their involvement in signal transduction they are also involved with cell proliferation, differentiation and function. PKCγ is found primarily in the brain and spinal cord. Expression in other tissues including epidermal, adrenal, haematopoietic, and some tumors is low or variable.
      HostMouse
      Immunogen speciesBovine
      Immunogenpurified bovine brain PKC
      Epitopewithin the hinge region of PKC
      CloneMC5
      IsotypeIgG2a
      Speciesbovine, human, mouse, rat
      Positive controlNIH3T3 cells or brain tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsMay be used to block the down regulation of PKC activity. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesXiao, H., et al. 1994. J. Neurosurg. 81, 734.
      Jin, L., et al. 1993. Am. J. Pathol. 142, 569.
      Nishikawa, M., and Shirakawa, S., 1992. Leuk. Lymphoma 8, 201.
      Wetsel, W.C., et al. 1992. J. Cell. Biol. 117, 121.
      Clark, E.A., et al. 1991. Lab. Invest. 64, 35.
      Hirabayashi, N., et al. 1990. Mol. Carcinog. 3, 171.
      Nishizuka, Y., 1989. Cancer 63, 1892.
      Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.
      Application referencesImmunoprecipitation Yong, S., et al. 1988. Eur. J. Biochem. 173, 247. Original Clone Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.