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ABE1877-25UL Anti-PAXIP1

Anti-PAXIP1, Cat. No. ABE1877-25UL, is a highly specific rabbit polyclonal antibody, that targets PAX-interacting protein 1 and has been tested in Western Blotting, Chromatin Immunoprecipitation (ChIP), ChIP-seq and Immunoprecipitation.

Anti-PAXIP1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Paxip1, Ptip UniProt Number: Q6NZQ4
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ABE1877-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ChIP, ChIP-seq, IPRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1877-25UL
      DescriptionAnti-PAXIP1
      Alternate Names
      • PAX-interacting protein 1
      • PAX transactivation activation domain-interacting protein
      • PAXIP
      Background InformationPAX-interacting protein 1 (UniProt: Q6NZQ4; also known as PAX transactivation activation domain-interacting protein, PAXIP1) is encoded by the Paxip1 (also known as Ptip) gene (Gene ID: 55982) in murine species. PAXIP1 is a nuclear matrix protein that is involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes such as the MLL2/MLL3 complex. In vitro it is shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks. It recruits Tp53bp1 to DNA damage foci and effective DNA damage response appears to require the association with Tp53bp1 phosphorylated by ATM. It also recruits PAGR1A (Pax-Interacting Protein 1-associated glutamate rich protein 1a) to DNA damage sites and PAGR1A:PAXIP1 complex is believed to be essential for cell survival. PAXIP1 expression is reported in all tissues and high expression is observed in embryonic kidney and brain. PAXIP1 contains 6 BRCT domains and BRCT domains 1 and 2 mediate its interaction with PAGR1A and interaction with MLL2/MLL3 is accomplished with BRCT domains 5 and 6. Mice with defective Paxip1 gene are developmentally retarded and usually face embryonic lethality by day 9.5 (E9.5).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-PAXIP1, Cat. No. ABE1877-25UL, is a highly specific rabbit polyclonal antibody, that targets PAX-interacting protein 1 and has been tested in Western Blotting, Chromatin Immunoprecipitation (ChIP), ChIP-seq and Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      • Immunoprecipitation
      Application NotesChromatin Immunoprecipitation Analysis: A representative lot detected PAXIP1 in mouse embryonic fibroblasts (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).
      ChIP-seq Analysis: Analysis: A representative lot detected PAXIP1 in ChIP-seq applications (Callen, E., et. al. (2012). Immunity. 37(6):971-85). Immunoprecipitation Analysis: A representative lot detected PAXIP1 in Immunoprecipitation applications (Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).
      Immunoprecipitation Analysis: 1 µg from a representative lot detected PAXIP1 in nuclear extract of wild type and PTIP KO mouse embryonic fibroblasts (C ourtesy of Dr. Ji-Eun Lee and Dr. Kai Ge at NIH).
      Western Blotting Analysis: A representative lot detected PAXIP1 in Western Blotting applications (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39; Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 199 amino acids from the N-terminal region of murine PAX-interacting protein 1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects PAX-interacting protein 1 in human and murine cells. It targets an epitope within 199 amino acids from the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • Paxip1
      • Ptip
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight51 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with with recombinant Pax-interacting protein 1.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant PAX-interacting protein 1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1877-25UL 04054839464737