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CB1045 Anti-OCLN Mouse pAb

This Anti-OCLN Mouse pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of OCLN.

Anti-OCLN Mouse pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Occludin

CB1045
  
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      Overview

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, MMPolyclonal Antibody
      Description
      OverviewRecognizes the ~55-60 kDa OCLN protein in HeLa cells and human and mouse liver tissue.
      Catalogue NumberCB1045
      Brand Family Calbiochem®
      SynonymsAnti-Occludin
      Application Data
      Detection of human OCLN by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse OCLN by immunoblotting. Sample: Whole tissue extract from mouse liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human OCLN by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1:1200) and an anti-CSNK1E mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHuman liver tissue, Mouse liver tissue, HeLa cells
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunocytochemistry (1:50-1:1200)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, human OCLN (aa 1-522)
      ImmunogenHuman
      HostMouse
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      CB1045 0