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MABE1043 Anti-Nup62 Antibody, clone 2A11

Anti-Nup62 Antibody, clone 2A11 is an antibody against Nup62 for use in Western Blotting and Immunocytochemistry.

Anti-Nup62 Antibody, clone 2A11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: NUP62 UniProt Number: P37198
MABE1043
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1043
      DescriptionAnti-Nup62 Antibody, clone 2A11
      Alternate Names
      • Nuclear pore glycoprotein p62
      • Nup62
      • 62 kDa nucleoporin
      • Nucleoporin Nup62
      Background InformationNuclear pore glycoprotein p62 (UniProt P37198; also known as 62 kDa nucleoporin, Nucleoporin Nup62) is encoded by the NUP62 (Also known as IBSN, SNDI) gene in human (Gene ID 23636). The nuclear pore complex (NPC) acts as a passageway for nucleocytoplasmic transport. Soluble cargo-protein complexes navigate through the pore by binding to phenylalanine-glycine (FG)-repeat proteins attached to the channel walls. The Nup62 complex is located in the center of the NPC and contains the FG-repeat proteins Nup62, Nup54, and Nup58 associated with each other via conserved coiled-coil segments in a 1:1:1 stiochiometric ratio. The nuclear envelope breaks down during prometaphase. The disassembled nucleoporin subcomplexes act as critical regulators of various mitotic events to ensure proper chromosome segregation. Specifically, Nup62 is reported to localize to centrosomes/spindle poles, where it associates with the centrosomal proteins gamma-tubulin and hSAS-6. Nup62 is involved in the recruitment of critical proteins to the centrosome and its depletion results in mislocalization of several centrosomal components.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-Nup62 Antibody, clone 2A11 is an antibody against Nup62 for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected Nup62 in HeLa cell nuclear membrane fraction (Fukuhara, T., et al. (2006). Hybridoma. 25(2):51-59).
      Immunocytochemistry Analysis: A representative lot detected Nup62 in HeLa cells (Fukuhara, T., et al. (2006). Hybridoma. 25(2):51-59).
      Immunocytochemistry Analysis: A representative lot detected Nup62 in HeLa S3 cells (Maeshima, K., et al. (2006). J. of Cell Science. 119:4442-4451).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to aa1-179 of human Nup62.
      EpitopeWithin aa1-179.
      Clone2A11
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NUP62
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~62 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa and Raji cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nup62 in 10 µg of HeLa and Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1043 04055977169850