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AB951-I Anti-Neuron Specific Enolase Antibody

Anti-Neuron Specific Enolase Antibody is a highly specific rabbit polyclonal antibody, that targets Neuron-Specific Enolase & has been tested in western blotting & IHC (Paraffin).

Anti-Neuron Specific Enolase Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: ENO2 UniProt Number: P09104
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AB951-I
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWB, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB951-I
      ReplacesAB951
      DescriptionAnti-Neuron Specific Enolase Antibody
      Alternate Names
      • Gamma-enolase
      • 2-phospho-D-glycerate hydro-lyase
      • Enolase 2
      • Neural enolase
      • Neuron-specific enolase
      • NSE
      Background InformationThe glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase, E.C. 4.2.1.11) is composed of two subunits and exists in three molecular forms in human brain: non-neuronal enolase (NNE; alpha, alpha'-enolase), neuron-specific enolase (NSE; gamma,gamma'-enolase, also known as 14-3-2 protein and NSP) and the short-lived heterodimer alpha, gamma'-enolase. The neuron specific form is a highly acidic homodimeric protin of 78kDa, which has been found in central and peripheral nervous elements and also in many neuroendocrine cells and derivative tumors. NSE is regarded as the best described serum marker for small cell lung cancer. In addition, the measurement of NSE levels in both serum and cerebrospinal fluid following cerebral ischaemia and traumatic head injury provides a reliable laboratory indicator of the degree of brain cell damage and may allow early prediction of prognostic outcome.
      Product Information
      FormatPurified
      Control
      • Rat brain tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neuron Specific Enolase Antibody is a highly specific rabbit polyclonal antibody, that targets Neuron-Specific Enolase & has been tested in western blotting & IHC (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a representative lot detected in Neuron Specific Enolase in human thalamus tissue and glioma sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the near N-terminus of human Neuron Specific Enolase.
      EpitopeNear N-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes a region near the N-terminus of Neuron Specific Enolase.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human, Rat, and Mouse. Predicted to react with Bovine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ENO2
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~47 kDa observed. Cat. No. AB951, Anti-Neuron Specific Enolase detects Alpha Enolase (ENO1) at ~39kDa. However, Neuron Specific Enolase has been observed at ~48 kDa (Braus, B., K., et al (2008). Vet Immunol Immunopathol 124(1-2):177-183.; Feo, S., et al. (2000). FEBS Lett. 473(1):47-52.).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Neuron Specific Enolase in 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      AB951-I 04053252916618