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AP1166 Anti-NGFRAP1 Mouse mAb (4E5)

This Anti-NGFRAP1 Mouse mAb (4E5) is validated for use in ELISA, Immunoblotting for the detection of NGFRAP1.

Anti-NGFRAP1 Mouse mAb (4E5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-NADE, Anti-BEX3, Anti-Nerve Growth Factor Receptor Associated Protein 1

AP1166
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes NGFRAP1 protein.
      Catalogue NumberAP1166
      Brand Family Calbiochem®
      SynonymsAnti-NADE, Anti-BEX3, Anti-Nerve Growth Factor Receptor Associated Protein 1
      Application Data
      Detection of human NGFRAP1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-NGFRAP1 Mouse mAb (4E5) (Cat. No. AP1166) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 1-110 human NGFRAP1, expressed as a GST fusion protein
      Clone4E5
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1166 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2010 RFH
      SynonymsAnti-NADE, Anti-BEX3, Anti-Nerve Growth Factor Receptor Associated Protein 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg)
      Application Data
      Detection of human NGFRAP1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-NGFRAP1 Mouse mAb (4E5) (Cat. No. AP1166) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the NGFRAP1 protein.
      BackgroundThe p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Coexpression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 1-110 human NGFRAP1, expressed as a GST fusion protein
      Clone4E5
      IsotypeIgG2b
      Specieshuman
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review