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OP13 Anti-N-Myc Mouse mAb (NCM II 100)

This Anti-N-Myc Mouse mAb (NCM II 100) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of N-Myc.

Anti-N-Myc Mouse mAb (NCM II 100) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP13
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

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OP13-100UG
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      Description
      OverviewRecognizes the N-myc protein and its cleavage product in IMR5 cells.
      Catalogue NumberOP13
      Brand Family Calbiochem®
      References
      ReferencesBrondyk, W.H. 1991. Oncogene 6, 1269.
      Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
      LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
      Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
      Slamon, D.J., et al. 1986. Science 232, 768.
      Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
      Brodeur, G.M., et al. 1984. Science 224, 1121.
      Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
      Kohl, N.E., et al. 1983. Cell 35, 603.
      Schwab, M., et al. 1983. Nature 305, 245.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Positive controlIMR5 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone, Immunofluorescence Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoblotting Brondyk, W.H. 1991. Oncogene 6, 1269. Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoprecipitation Brondyk, W.H. 1991. Oncogene 6, 1269.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunofluorescence (1-5 µg/ml, see application references)
      Immunoprecipitation (1-2 µg/sample, see application references)
      Application CommentsIt is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human, N-myc fusion protein, expressed in E. coli
      ImmunogenHuman
      CloneNCM II 100
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP13-100UG 04055977224795

      Documentation

      Anti-N-Myc Mouse mAb (NCM II 100) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-N-Myc Mouse mAb (NCM II 100) Certificates of Analysis

      TitleLot Number
      OP13

      References

      Reference overview
      Brondyk, W.H. 1991. Oncogene 6, 1269.
      Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
      LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
      Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
      Slamon, D.J., et al. 1986. Science 232, 768.
      Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
      Brodeur, G.M., et al. 1984. Science 224, 1121.
      Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
      Kohl, N.E., et al. 1983. Cell 35, 603.
      Schwab, M., et al. 1983. Nature 305, 245.

      Citations

      Title
    • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunofluorescence (1-5 µg/ml, see application references)
      Immunoprecipitation (1-2 µg/sample, see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc and its cleavage products.
      BackgroundThe v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human, N-myc fusion protein, expressed in E. coli
      CloneNCM II 100
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlIMR5 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsIt is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBrondyk, W.H. 1991. Oncogene 6, 1269.
      Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
      LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
      Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
      Slamon, D.J., et al. 1986. Science 232, 768.
      Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
      Brodeur, G.M., et al. 1984. Science 224, 1121.
      Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
      Kohl, N.E., et al. 1983. Cell 35, 603.
      Schwab, M., et al. 1983. Nature 305, 245.
      Citation
    • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
      		•
      Application referencesOriginal Clone, Immunofluorescence Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoblotting Brondyk, W.H. 1991. Oncogene 6, 1269. Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoprecipitation Brondyk, W.H. 1991. Oncogene 6, 1269.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies