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205605 Anti-N-Cadherin Rat mAb (NCD-2)

Anti-N-Cadherin Rat mAb (NCD-2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Key Spec Table

      Host
      R
      Description
      Overview

      This product has been discontinued.





      Recognizes the N-cadherin protein. May be used to inhibit E-cadherin-dependent cell-cell contact.
      Catalogue Number205605
      Brand Family Calbiochem®
      References
      ReferencesTakeichi, M. 1991. Science 251, 1451.
      Fujimori, T., et al. 1990. Development 110, 97.
      Hatta, K., et al. 1988. J. Cell Biol. 106, 873.
      Matsunaga, M., et al. 1988. Neuron 1, 289.
      Takeichi, M. 1988. Development 102, 639.
      Hatta, K., et al. 1987. Dev. Biol. 120, 215.
      Hatta, K., and Takeichi, M. 1986. Nature 320, 447.
      Product Information
      FormLyophilized
      FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
      Applications
      Key Applications Cell Inhibition Assays
      Frozen Sections
      Immunoblotting (Western Blotting)
      Not Paraffin Sections
      Application NotesCell Inhibition Assays (100 µg/ml)
      Immunoblotting (10 µg/ml)
      Immunohistochemistry (10 µg/ml)
      Frozen Sections (10 µg/ml)
      Paraffin Sections (not recommended)
      Application CommentsSpecific for chicken and frog N-cadherin. Note: 10 mM Ca2+ should be present in all buffers to stabilize the cadherin antigen. The antibody may be used to inhibit N-cadherin-dependent cell-cell contact. Not suitable for use with paraffin-embedded sections, when used in immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogennervous tissue of chick embryos
      ImmunogenChick Embryo
      CloneNCD-2
      HostRat
      IsotypeIgG2a
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Further dilute in 20 mM TBS, pH 7.4, 10 mM CaCl₂, and 1% BSA. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Avoid freeze/thaw cycles of solution. TBS is recommended as PBS causes precipitation.
      Global Trade Item Number
      Catalogue Number GTIN
      205605 0