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ABE1851-25UG Anti-MLL-3

Anti-MLL3, Cat. No. ABE1851, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase 2C and has been tested in Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin), and Western Blotting.

Anti-MLL-3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Kmt2C, Mll3 UniProt Number: Q8BRH4
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ABE1851-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RChIP, IH(P), WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1851-25UG
      DescriptionAnti-MLL-3
      Alternate Names
      • Histone-lysine N-methyltransferase 2C
      • EC: 2.1.1.43
      • Lysine-N-methyltransferase 2C
      • Myeloid-lineage leukemia protein 3 homolog
      Background InformationHistone-lysine N-methyltransferase 2C (UniProt: Q8BRH4; also known as EC: 2.1.1.43, Lysine-N-methyltransferase 2C, Myeloid-lineage leukemia protein 3 homolog, MLL-3) is encoded by the Kmt2c (also known as Mll3) gene in murine species. Mll3 gene is a member of the myeloid/lymphoid or mixed-lineage leukemia family. MLL-3 protein is a DHHC-type zinc finger protein with six PHD-type zinc fingers, a SET domain, a post-SET domain and a RING-type zinc finger. It methylates Lys-4 of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. MLL-3 is a central component of the MLL2/3 complex, a coactivator complex of nuclear receptors, involved in transcriptional co-activation. In adults, MLL-3 is detected in testis, kidney, spleen and lung and is weakly expressed in brain. It is absent in heart and liver tissue. MLL-3 has been reported to play a role in adipogenesis.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MLL3, Cat. No. ABE1851, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase 2C and has been tested in Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MLL-3 in mouse testis, rat uterus, and mouse kidney tissues.

      Chromatin Immunoprecipitation Analysis: A representative lot detected MLL-3 in MEF cells (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8).

      Western Blotting Analysis: A representative lot detected MLL-3 in Western Blotting applications (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8); Bres, V., et. al. (2009). Mol Cell. 36(1):75-87).

      Immunohistochemistry Analysis: A representative lot detected MLL-3 in mouse urothelial tumors (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8).
      Biological Information
      ImmunogenA linear peptide corresponding to 18 amino acids from the N-terminal region of human MLL-3.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antiody detects MLL-3 protein in human, mouse and rat cells. It targets and epitope within 18 amino acids from the internal region of N-terminal half.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • Kmt2C
      • Mll3
      Purification MethodAffinity Purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U2OS nuclear extract.
      Western Blotting Analysis: 1 µg/mL of this antibody detected MLL-3 in 10 µg of U2OS cell nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1851-25UG 04054839438028