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MABE1824-25UG Anti-LbCpf1 Antibody, clone 2D5-6G11

Anti-LbCpf1, clone 2D5-6G11 Antibody, Cat. No. MABE1824, is a highly specific mouse monoclonal antibody that targets LbCpf1 and has been tested for use in Western Blotting.

Anti-LbCpf1 Antibody, clone 2D5-6G11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function
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MABE1824-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      BactWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1824-25UG
      DescriptionAnti-LbCpf1 Antibody, clone 2D5-6G11
      Alternate Names
      • Cpf1
      Background InformationCRISPR-associated endonuclease Cas12a (UniProt: A0A182DWE3; also known as CRISPR-associated endonuclease Cpf1; Lbcpf1) is a single crRNA-guided endonuclease of the class 2 CRISPR-Cas system that lacks tracrRNA and undergoes large conformational changes upon crRNA binding. Cpf1 has several features that can distinguish it from Cas9. Unlike Cas9 that uses dual-guide RNAs, Cpf1 is guided by a crRNA. It recognizes a T-rich PAM (T-rich protospacer adjacent motif), whereas Cas9 favors a G-rich PAM and Cpf1 generates staggered ends at its PAM-distal region, but Cas9 creates blunt ends at its PAM-proximal region. Cpf1 displays higher targeting specificity in mammalian cells when compared to Cas9. It has been shown that depending on the PAM sequences, LbCpf1 can undergo conformational changes to form altered interactions with the PAM-containing DNA duplexes, hence achieving the relaxed PAM recognition. (Ref.: Zetsche, B., et al. (2015). Cell 163(3); 759-77; Yamano, T., et al. (2017). Mol. Cell 67(4); 633-645; Gao, P., et al. (2016). Cell Res. 26(8); 901-913).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-LbCpf1, clone 2D5-6G11 Antibody, Cat. No. MABE1824, is a highly specific mouse monoclonal antibody that targets LbCpf1 and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: Various dilutions from a representative lot detected LbCpf1 in lysates from HEK293 cells expressing HA-tagged LbCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
      Biological Information
      ImmunogenHis-tagged recombinant fragment from the N-terminal region of Lachnospiracea bacteria.
      EpitopeN-terminus
      Clone2D5-6G11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2D5-6G11 specifically detects CRISPR-associated endonuclease Cas12a (LbCpf1).
      IsotypeIgG1κ
      Species Reactivity
      • Bacteria
      Species Reactivity NoteLachnospiracea.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Molecular Weight~144 kDa observed; 143.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cells expressing HA-tagged LbCpf1.

      Western Blotting Analysis: 1 µg/mL of this antibody detected LbCpf1 in lysate from HEK293 cells expressing HA-tagged LbCpf1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1824-25UG 04054839558047