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MAB131871 Anti-KLH Antibody, clone 3B1

Anti-KLH, clone 3B1, Cat. No. MAB131871, is a mouse monoclonal antibody that detects Keyhole limpet hemocyanin and has been tested for use in Western Blotting.

Anti-KLH Antibody, clone 3B1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology UniProt Number: Q10583, P81732 and Q10584
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MAB131871
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MlWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB131871
      DescriptionAnti-KLH Antibody, clone 3B1
      Alternate Names
      • Keyhole Limpet Hemocyanin
      Background InformationSubstances with low molecular weights are generally not immunogenic and require the aid of carrier proteins to stimulate an immune response. Carrier proteins have relatively high molecular weights and carry many epitopes. An immune response to a hapten-carrier conjugate results in antibodies that are specific for both the hapten and the carrier. Hemocyanin is a high molecular weight respiratory metalloprotein found in the hemolymph of many mollusks and crustaceans. Molluscan hemocyanin is based on an approximately 400 kDa polypeptide subunit that is folded into a chain of multiple, immunologically distinct domains, each with a binuclear copper site. Keyhole Limpet Hemocyanin (KLH), the hemocyanin from the giant keyhole limpet, Megathura crenulata, is the most widely used carrier protein. It is an extremely large protein (6 - 7.5 million daltons) that can exist as a di-decamer, multi-decamers, or flexible tubules of varying length. It is easily dissociated into subunits and the dissociation-association reaction depends on protein concentration, ionic strength, and pH. At pH 6.5, KLH exists in a fully associated state without any evidence of dissociation products. In contrast, at pH 8.5 in borate saline the molecule is completely dissociated.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-KLH, clone 3B1, Cat. No. MAB131871, is a mouse monoclonal antibody that detects Keyhole limpet hemocyanin and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPurified Keyhole limpet hemocyanin from Megathura crenulata
      Clone3B1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3B1 detects keyhole limpet hemocyannin (KLH) and KLH conjugated to peptide or protein.
      IsotypeIgMκ
      Species Reactivity
      • Mollusk
      Species Reactivity NoteMollusk. Reacts with Megathura crenulata.
      Antibody TypeMonoclonal Antibody
      UniProt Number
      Molecular Weight~375 kDa observed; Calculated ~ 6 to 7.5 MDa (undissociated). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with Keyhole Limpet Hemocyanin (KLH).

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Keyhole Limpet Hemocyanin.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB131871 04054839416606