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ABE2588-25UL Anti-H3Q5dopaminyl

Anti-H3Q5dopaminyl, Cat. No. ABE2588, is a rabbit polyclonal antibody that detects Histone H3 dopaminylated on glutamine 5 and is tested for use in Dot Blot, Peptide Inhibition Assay, and Western Blotting.

ABE2588-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE2588-25UL
      DescriptionAnti-H3Q5dopaminyl
      Alternate Names
      • Dopaminylated Histone H3
      • H3Q5dopaminylated
      Background InformationHistone H3.1t (UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication. Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone 3.1 is also shown to undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. It has been reported that Histone H3 can also undergo dopaminylation ((H3Q5dop), which can potentiate the binding of adjacent histone post-translational modification interacting proteins and play a vital role in dopaminergic neuronal transcription. This rabbit polyclonal antibody specifically binds to Histone H3 dopaminylated on glutamine 5. (Ref.: Lepack, AE., et al (2020). Science. 368(6487); 197-201).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-H3Q5dopaminyl, Cat. No. ABE2588, is a rabbit polyclonal antibody that detects Histone H3 dopaminylated on glutamine 5 and is tested for use in Dot Blot, Peptide Inhibition Assay, and Western Blotting.
      Key Applications
      • Dot Blot
      • Peptide Inhibition Assay
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:200 dilution from a representative lot detected H3Q5dopaminyl in human ventral tegmental area (Courtesy of Ian Maze Lab; Icahn School of Medicine at Mount Sinai, New York).

      Dot Blot Analysis: A representative lot detected H3Q5dopaminyl in Dot Blot applications (Lepack, AE., et al. (2020). Science. 368(6487); 197 201).

      Peptide Inhibition Assay: A representative lot detected H3Q5dopaminyl in Peptide Inhibition Assay applications ( Lepack, AE., et al. (2020). Science. 368(6487); 197 201).

      Western Blotting Analysis: A representative lot detected H3Q5dopaminyl in Western Blotting applications. (Lepack, AE., et al. (2020). Science. 368(6487); 197 201).

      Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected H3Q5dopaminyl in Unmodified H3Q5 peptide vs. H3Q5dopaminyl peptide (Courtesy of Ian Maze Lab; Icahn School of Medicine at Mount Sinai, New York).



      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3 with dopaminylated glutamine 5.
      EpitopeN-terminus
      Concentration0.03 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Histone H3 dopaminylated on glutamine 5.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~17 kDa observed; 15.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant H3 dopaminyated on glutamine 5.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant Histone H3 dopaminylated on glutamine 5 with 2.5 mM dopamine, but not with one treated with transglutaminase 2 (0.5 mg).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2588-25UL 04061842414895

      Documentation

      Anti-H3Q5dopaminyl MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-H3Q5dopaminyl Certificates of Analysis

      TitleLot Number
      Anti-H3Q5dopaminyl - 3684698 3684698
      Anti-H3Q5dopaminyl - 3689468 3689468
      Anti-H3Q5dopaminyl - 3695325 3695325
      Anti-H3Q5dopaminyl - 3700374 3700374
      Anti-H3Q5dopaminyl - 3715675 3715675
      Anti-H3Q5dopaminyl - 3719550 3719550
      Anti-H3Q5dopaminyl - 3720089 3720089
      Anti-H3Q5dopaminyl - 3726522 3726522
      Anti-H3Q5dopaminyl - 3729955 3729955
      Anti-H3Q5dopaminyl - 3734501 3734501
      Show more

      References

      Reference overviewPub Med ID
      Dopaminylation of histone H3 in ventral tegmental area regulates cocaine seeking
      Ashley E Lepack 1 , Craig T Werner 2 , Andrew F Stewart 1 , Sasha L Fulton 1 , Ping Zhong 3 , Lorna A Farrelly 1 , Alexander C W Smith 1 , Aarthi Ramakrishnan 1 , Yang Lyu 1 , Ryan M Bastle 1 , Jennifer A Martin 2 , Swarup Mitra 2 , Richard M O'Connor 1 , Zi-Jun Wang 3 , Henrik Molina 4 , Gustavo Turecki 5 , Li Shen 1 , Zhen Yan 3 , Erin S Calipari 6 , David M Dietz 2 , Paul J Kenny 1 , Ian Maze
      Science  368(6487)  197-201  2020

      Show Abstract       		32273471 32273471