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GR32L Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2)

Anti-Glucocorticoid Receptor (Ab-2), mouse monoclonal, clone BuGR2, recognizes ~94-97 kDa glucocorticoid receptor in mouse liver. It is validated for WB, ICC, IP & IHC (Frozen & paraffin sections).

Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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GR32L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Gp, M, Rb, R, ShMMonoclonal Antibody

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GR32L-100UG
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      Description
      OverviewRecognizes the ~94-97 kDa glucocorticoid receptor in mouse liver tissue.
      Catalogue NumberGR32L
      Brand Family Calbiochem®
      Application Data
      Detection of mouse glucocorticoid receptor by immunoblotting. Sample: Whole tissue extract from mouse liver. Primary antibody: Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2) (Cat No. GR32L) (5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesHussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
      Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev. 18 306.
      Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
      Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
      Weigel, N.L. 1996. Biochem J. 319, 657.
      Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
      Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.
      Product Information
      FormLyophilized
      FormulationLyophilized from PBS, pH 7.2.
      Positive controlmouse liver extract
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Gametchu, B. and Harrison, R., 1984. Endocrinology 114, 274.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (5 µg/ml)
      Immunoblotting (5 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (see comments)
      Paraffin Sections (5 µg/ml)
      Application CommentsDoes not cross react with androgen, progesterone, estrogen or mineralocorticoid receptors. Enzymatic digestion and immunoblot analysis has shown that this antibody reacts with the undigested 97 kDa glucocorticoid receptor, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid and DNA-binding chymotrypsin fragment. Immunocytochemical localization of the glucocorticoid receptor is observed in the cytoplasm and nucleus, even in the presence of hormone. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified, rat glucocorticoid receptor
      ImmunogenRat
      CloneBuGR2
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Guinea Pig
      • Mouse
      • Rabbit
      • Rat
      • Sheep
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with 0.1 ml sterile PBS, pH 7.2. Be careful to reconstitute the entire contents of the vial; during shipment and handling, portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GR32L-100UG 04055977228298

      Documentation

      Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2) Certificates of Analysis

      TitleLot Number
      GR32L

      References

      Reference overview
      Hussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
      Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev. 18 306.
      Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
      Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
      Weigel, N.L. 1996. Biochem J. 319, 657.
      Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
      Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      ApplicationFrozen Sections (5 µg/ml)
      Immunoblotting (5 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (see comments)
      Paraffin Sections (5 µg/ml)
      Application Data
      Detection of mouse glucocorticoid receptor by immunoblotting. Sample: Whole tissue extract from mouse liver. Primary antibody: Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2) (Cat No. GR32L) (5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-AgX-653 myeloma cells (see application references). Recognizes the ~94-97 kDa glucocorticoid receptor.
      BackgroundSteroids regulate important neuronal functions including the organization of neural circuits during development and the regulation of synapse organization in the adult. Steroid hormones may promote cell survival and differentiation of both neurons and glial cells, regulate synaptogenesis, prevent synapse elimination, cause axonal and dendritic growth, and play a role during regeneration of the nervous system. Steroid hormone receptors are members of a large family of nuclear ligand-activated transcription factors that includes the androgen, estrogen, glucocorticoid, and progesterone receptors. In the absence of hormone, steroid receptors are associated in complexes with heat shock proteins and immunophilins. Steroid binding results in the dissociation of the heat-shock protein, receptor dimerization, and binding to specific hormone response elements (HRE) to produce a transcriptionally active complex that leads to the activation or repression of target genes. Steroid receptors are regulated by phosphorylation, however, modulation of kinase activity can also cause receptor activation in the absence of hormone. Recent evidence suggests that steroid receptor expression can be regulated by neurotrophins, and that steroids can regulate the expression of trophic factors and their receptors.
      HostMouse
      Immunogen speciesRat
      Immunogenpartially purified, rat glucocorticoid receptor
      CloneBuGR2
      IsotypeIgG2a
      Speciesnot amphibian, not avian, guinea pig, mouse, not primate, rabbit, rat, sheep
      Positive controlmouse liver extract
      FormLyophilized
      FormulationLyophilized from PBS, pH 7.2.
      Preservative≤0.1% sodium azide
      CommentsDoes not cross react with androgen, progesterone, estrogen or mineralocorticoid receptors. Enzymatic digestion and immunoblot analysis has shown that this antibody reacts with the undigested 97 kDa glucocorticoid receptor, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid and DNA-binding chymotrypsin fragment. Immunocytochemical localization of the glucocorticoid receptor is observed in the cytoplasm and nucleus, even in the presence of hormone. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with 0.1 ml sterile PBS, pH 7.2. Be careful to reconstitute the entire contents of the vial; during shipment and handling, portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesHussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
      Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev. 18 306.
      Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
      Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
      Weigel, N.L. 1996. Biochem J. 319, 657.
      Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
      Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.
      Application referencesOriginal Clone Gametchu, B. and Harrison, R., 1984. Endocrinology 114, 274.