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ABS1601 Anti-GRASP65 Antibody

Anti-GRASP65 Antibody is an antibody against GRASP65 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.

Anti-GRASP65 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: Gorasp1, Grasp65 UniProt Number: O35254
ABS1601
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IP, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1601
      DescriptionAnti-GRASP65 Antibody
      Alternate Names
      • Golgi reassembly-stacking protein 1
      • GRASP65
      • Golgi peripheral membrane protein p65
      • Golgi reassembly-stacking protein of 65 kDa
      Background InformationGolgi reassembly stacking protein of 65 kDa (also called Golgi reassembly and stacking protein 1 or Golgi peripheral membrane protein p65) is encoded by the Grasp65 or Gorasp65 gene in rat (GRASP65, GORASP1, or GOLPH5 gene in human). It is is a Golgi structural protein and mediates cisternae stacking via self-aggregation as well as interactions with other Golgi membrane-docking proteins, such as GM130. GRASP65 dimers are shown to aggregate in vitro and form extensive trans oligomers, which disaggregate upon phosphorylation by cdc2-cyclin B and polo-like kinases, and re-aggregate upon dephosphorylation (PMID 12839990). Fluorescent imaging studies reveal a dynamic exchange between the membrane-associated and cytosolic GRASP65. (PMID 11706049).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum containing 0.05% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-GRASP65 Antibody is an antibody against GRASP65 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated GRASP65 in APPswe/PS1ΔE9 cells stably expressing GFP-tagged GRASP65 WT (Joshi, G., et al. (2013). PNAS. 1-10).
      Immunocytochemistry Analysis: A representative lot detected GRASP65 in NRK cells (Tang, D., et al. (2012). Biology Open. 1204-1214).
      Immunocytochemistry Analysis: A representative lot detected GRASP65 in CHO cells transfected with WT APP and PS1 or APPswe/PS1ΔE9 mutants, cultured primary hippocampal neurons, and WT CHO cells (Joshi, G., et al. (2013). PNAS. 1-10).
      Western Blotting Analysis: A representative lot detected GRASP65 in APPswe/PS1ΔE9 cells stably expressing GFP-tagged GRASP65 WT, cells stably expressing GFP-tagged GRASP65 WT, and mouse brain (Joshi, G., et al. (2013). PNAS. 1-10).
      Biological Information
      ImmunogenGST-tagged Recombinant protein corresponding to rat GRASP65.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gorasp1
      • Grasp65
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~65 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human alzheimer's brain tissue lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GRASP65 in 10 µg of human alzheimer's brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1601 04055977161908

      Documentation

      Anti-GRASP65 Antibody Certificates of Analysis

      TitleLot Number
      Anti-GRASP65 -Q2559158 Q2559158