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324390 Anti-Dopamine D₁ Receptor Rabbit pAb

Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

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324390-100UL
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      Description
      OverviewRecognizes the dopamine D1 receptor in rat brain. Does not cross-react with other dopamine receptors.
      Catalogue Number324390
      Brand Family Calbiochem®
      References
      ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlRat brain
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting and Immunohistochemistry Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325. Species Reactivity Kipnis, J., et al. 2004. Journal of Neuroscience 24 6133.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Free-floating Sections
      Frozen Sections
      Immunoblotting (Western Blotting)
      Not Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1:1000)
      Frozen Sections (1:5000)
      Free-Floating Sections (1:5000)
      Paraffin Sections (not recommended)
      Application CommentsThis antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
      Global Trade Item Number
      Catalogue Number GTIN
      324390-100UL 04055977216516