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MABT1349-25UG Anti-Desmoglein-2 Antibody, clone CSTEM28

Anti-Desmoglein-2 , clone CSTEM28, Cat. No. MABT1349, is a mouse monoclonal antibody that detects Desmoglein-2 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

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MABT1349-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1349-25UG
      DescriptionAnti-Desmoglein-2 Antibody, clone CSTEM28
      Alternate Names
      • Cadherin family member 5
      • HDGC
      Background InformationDesmoglein-2 (UniProt: Q14126; also known as Cadherin family member 5, HDGC) is encoded by the DSG2 (also known as CDHF5) gene (Gene ID: 1829) in human. Desmogleins are protein substances located in and on the surface of keratinocytes. They are present in specialized cell-cell adhesion structures enriched in tissues that undergo substantial mechanical stress. These proteins have been shown to be a critical factor in cell-to-cell adhesion. Desmoglein-2 is a single-pass type I membrane glycoprotein that is found in all tissues and in carcinomas. It serves as a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 24-49) that are subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 50-609), a transmembrane domain (aa 610-634), and a cytoplasmic domain (aa 635-1118). Mutations in DSG2 gene have been linked to familial type of arrhythmogenic right ventricular dysplasia that is characterized y infiltration of adipose and fibrous tissue into the right ventricle and loss of myocardial cells, resulting in ventricular and supraventricular arrhythmias. Desmoglein-2 levels are reported to be consistently upregulated in most cancers where it participates in the maintenance of intracellular junctions that prevents host immune responses. (Ref.: O Brien, CM., et al. (2017). Stem Cells. 35(3); 626-640).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Desmoglein-2 , clone CSTEM28, Cat. No. MABT1349, is a mouse monoclonal antibody that detects Desmoglein-2 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected Desmoglein-2 in Flow Cytometry (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

      Immunocytochemistry Analysis: A representative lot detected Desmoglein-2 in Immunocytochemistry (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

      ELISA Analysis: A representative lot detected Desmoglein-2 in ELISA (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to the extracellular domain of human Desmoglein-2.
      EpitopeExtracellular domain
      CloneCSTEM28
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone CSTEM28 is a mouse monoclonal antibody that detects Desmoglein-2. It targets an epitope within the extracellular domain.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DSG2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~78 kDa observed for recombinant fragment, 122.29 kDa calculated for full-length protein. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant human Desmoglein-2.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human Desmoglein-2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1349-25UG 04065268529058