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MABS2036 Anti-Cytochrome b Antibody, clone 5B3-6E3

Anti-Cytochrome b, clone 5B3-6E3, Cat. No. MABS2036, is a mouse monoclonal antibody that detects Complex III Cytochrome b and has been tested for use in Western Blotting.

Anti-Cytochrome b Antibody, clone 5B3-6E3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: MT-CYB, COB, CYTB, MTCYB UniProt Number: P00156
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MABS2036
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS2036
      DescriptionAnti-Cytochrome b Antibody, clone 5B3-6E3
      Alternate Names
      • Cytochrome b (UniProt: P00156
      • also known as Complex III subunit 3
      • Complex III subunit III
      • Cytochrome b-c1 complex subunit 3
      • Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
      Background InformationCytochrome b (UniProt: P00156; also known as Complex III subunit 3, Complex III subunit III, Cytochrome b-c1 complex subunit 3, Ubiquinol-cytochrome-c reductase complex cytochrome b subunit) is encoded by the MT-CYB (also known as COB, CYTB, MTCYB) gene (Gene ID: 4519) in human. Cytochrome b is a mitochondrial inner membrane protein that is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex). It is a part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c and contributes to the generation of a proton gradient across the mitochondrial membrane, which is used for ATP synthesis. The MT-CYB gene is a good phylogenetic marker. Defects in MT-CYB gene are a rare cause of mitochondrial dysfunction underlying different myopathies, which include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy, and sporadic mitochondrial myopathy. Some cases of Leber hereditary optic neuropathy (LHON) are also linked to defects in MT-CYB gene, which result in acute or subacute loss of central vision, due to optic nerve dysfunction.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cytochrome b, clone 5B3-6E3, Cat. No. MABS2036, is a mouse monoclonal antibody that detects Complex III Cytochrome b and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.1 µg/mL from a representative lot detected Cytochrome b in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA. (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR USA)
      Biological Information
      ImmunogenKLH-conjugated Synthetic peptide corresponding to the N-terminus of human cytochrome b.
      EpitopeN-terminus
      Clone5B3-6E3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5B3-6E3 detects cytochrome b in human mitochondria. It targets an epitope within the N-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MT-CYB
      • COB
      • CYTB
      • MTCYB
      Purification MethodProtein L
      UniProt Number
      Molecular Weight~28 kDa observed; 42.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected Cytochrome b in Mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2036 04054839355578