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ABE1010 Anti-Chromobox protein homolog 5 Antibody

This Anti-Chromobox protein homolog 5 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Chromobox protein homolog 5.

Anti-Chromobox protein homolog 5 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: CBX5, HP1A UniProt Number: P45973
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ABE1010
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1010
      DescriptionAnti-Chromobox protein homolog 5 Antibody
      Alternate Names
      • Chromobox protein homolog 5
      • Antigen p25
      • Heterochromatin protein 1 homolog alpha
      • HP1 alpha
      Background InformationChromobox protein homolog 5, also known as Antigen p25, Heterochromatin protein 1 homolog alpha (HP1 alpha), and encoded by the gene name CBX5 or HP1A is a component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, Chromobox protein homolog 5 is excluded from chromatin when 'Tyr-41' of histone H3 (H3Y41ph) is phosphorylated. CBX5 is implied to interact with lamin-B receptor (LBR), which can contribute to the association of the heterochromatin with the inner nuclear membrane. Recent studies have shown that CBX5 is involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Chromobox protein homolog 5 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Chromobox protein homolog 5.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected Chromobox protein homolog 5 in HeLa and A431 cells.
      Alexa Fluor® is a registered trademark of Life Technologies®.
      Biological Information
      ImmunogenBCP-conjugated linear peptide corresponding to human Chromobox protein homolog 5.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Bovine, Canine, Rhesus Macaque, Gorilla, Giant Panda based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CBX5
      • HP1A
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~23 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected Chromobox protein homolog 5 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1010 04053252981845