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CC43 Anti-Cdh1 (Ab-2) Mouse mAb (DH01)

Anti-Cdh1 (Ab-2), mouse monoclonal, clone DH01, recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells. It is validated for Western blotting and immunoprecipitation.

Anti-Cdh1 (Ab-2) Mouse mAb (DH01) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CC43
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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CC43-100UG
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      Description
      OverviewRecognizes the ~55 kDa Cdh-1 protein in Rat-1 cells.
      Catalogue NumberCC43
      Brand Family Calbiochem®
      Application Data
      Detection of human Cdh1 by immunoblotting. Sample: Human Cdh1. Primary antibody: Anti-Cdh1 (Ab-2) Mouse mAb (DH01) (Cat. No. CC43) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesKramer, E.R., et al. 2000 Mol. Biol. Cell 11, 1555.
      Petersen, B.O., et al. 2000 Genes Dev. 14, 2330.
      Pfleger, C.M., et al. 2001 Genes Dev. 15, 1759.
      Sorensen, C.S., et al. 2001 Mol. Cell Biol. 21, 3692.
      Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA 96, 11317.
      Visintin, R., et al. 1997 Science 278, 460.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Negative controlNormal mouse serum
      Positive controlJunkat or LS174T cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml)
      Immunoprecipitation (2 µg/mg protein lysate)
      Application CommentsDoes not cross-react with other WD repeat containing proteins, including Cdc20. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant human Cdh1 protein
      ImmunogenHuman
      CloneDH01
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CC43-100UG 04055977228472

      Documentation

      Anti-Cdh1 (Ab-2) Mouse mAb (DH01) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cdh1 (Ab-2) Mouse mAb (DH01) Certificates of Analysis

      TitleLot Number
      CC43

      References

      Reference overview
      Kramer, E.R., et al. 2000 Mol. Biol. Cell 11, 1555.
      Petersen, B.O., et al. 2000 Genes Dev. 14, 2330.
      Pfleger, C.M., et al. 2001 Genes Dev. 15, 1759.
      Sorensen, C.S., et al. 2001 Mol. Cell Biol. 21, 3692.
      Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA 96, 11317.
      Visintin, R., et al. 1997 Science 278, 460.

      Citations

      Title
    • Kyung Uk Hong, et al. (2007) Functional importance of the anaphase-promoting complex-Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis. Molecular and Cellular Biology 27, 3667-3681.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-August-2007 RFH
      ApplicationImmunoblotting (1 µg/ml)
      Immunoprecipitation (2 µg/mg protein lysate)
      Application Data
      Detection of human Cdh1 by immunoblotting. Sample: Human Cdh1. Primary antibody: Anti-Cdh1 (Ab-2) Mouse mAb (DH01) (Cat. No. CC43) (1 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~55 kDA Cdh1 protein.
      BackgroundCdh1, a homolog of the yeast Cdc20, is a ~55 kDa protein required for the degradation of anaphase-promoting complex (APC)-dependent proteolysis. Cdh1 acts as a limiting, substrate-specific activator of APC-dependent proteolysis and it is required for the degradation of APC substrates such as Clb2 and Ase1. Cdh1 plays an important role in mitotic regulation.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant human Cdh1 protein
      CloneDH01
      IsotypeIgG₁
      Specieshuman
      Positive controlJunkat or LS174T cells
      Negative controlNormal mouse serum
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with other WD repeat containing proteins, including Cdc20. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesKramer, E.R., et al. 2000 Mol. Biol. Cell 11, 1555.
      Petersen, B.O., et al. 2000 Genes Dev. 14, 2330.
      Pfleger, C.M., et al. 2001 Genes Dev. 15, 1759.
      Sorensen, C.S., et al. 2001 Mol. Cell Biol. 21, 3692.
      Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA 96, 11317.
      Visintin, R., et al. 1997 Science 278, 460.
      Citation
    • Kyung Uk Hong, et al. (2007) Functional importance of the anaphase-promoting complex-Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis. Molecular and Cellular Biology 27, 3667-3681.
      		•