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IM27L Anti-Cathepsin B (Ab-1) Mouse mAb (CA10)

Anti-Cathepsin B (Ab-1), mouse monoclonal, clone CA10, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in HepG2 cells. It is validated for WB, IF, and IP.

Anti-Cathepsin B (Ab-1) Mouse mAb (CA10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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IM27L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, PorcineMMonoclonal Antibody

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IM27L-100UG
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      Description
      OverviewRecognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in HepG2 cells.
      Catalogue NumberIM27L
      Brand Family Calbiochem®
      References
      ReferencesSloane, B.F. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152.
      Barret, A.J. 1987. Trends Biochem. Sci. 12, 193.
      Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 7721.
      Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol. 80, 535.
      Product Information
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicabonate buffer, 100 µg BSA (100 µg size) or 20 µg BSA (20 µg size).
      Positive controlHep G2 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (250 ng/ml)
      Immunoprecipitation (1 µg/ml)
      Immunofluorescence (see comments)
      Application CommentsThis antibody recognizes an epitope on the heavy chain of mature cathepsin B and therefore detects both mature and pro-cathepsin B. Also reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified human liver cathepsin B
      ImmunogenHuman
      Epitopewithin the heavy chain of mature cathepsin B
      CloneCA10
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Human
      • Porcine
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IM27L-100UG 04055977220513

      Documentation

      Anti-Cathepsin B (Ab-1) Mouse mAb (CA10) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cathepsin B (Ab-1) Mouse mAb (CA10) Certificates of Analysis

      TitleLot Number
      IM27L

      References

      Reference overview
      Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152.
      Barret, A.J. 1987. Trends Biochem. Sci. 12, 193.
      Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 7721.
      Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol. 80, 535.

      Citations

      Title
    • A. Grimaldi, et al. (2004) Role of cathepsin B in leech wound healing. Invertebrate Survival Journal 1, 38-46.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      ApplicationImmunoblotting (250 ng/ml)
      Immunoprecipitation (1 µg/ml)
      Immunofluorescence (see comments)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O-NS1 plasmacytoma cells. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.
      BackgroundCathepsin B (CB) is a normal lysosomal proteinase which is expressed in all cells. It is a cysteine endoproteinase and is structurally and functionally related to the papain family of proteinases. CB is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 623 amino acid propeptide, is activated to the single-chain form of 31 kDa or the two-chain form of 25 and 5 kDa subunits. Mature CB is localized in the lysosomes where it functions in protein turnover but has also been shown to be capable of degrading extracellular matrix proteins at acidic and neutral pH. Endogenous inhibitors of cysteine proteinases, the cystatins and stefins, may play a major role in regulating proteinase activity. In tumor cells and in cells exposed to mitogens, CB displays altered cellular trafficking resulting in the secretion of the 43 kDa precursor. Elevated levels of CB correlate with malignancy, suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.
      HostMouse
      Immunogen speciesHuman
      Immunogenpurified human liver cathepsin B
      Epitopewithin the heavy chain of mature cathepsin B
      CloneCA10
      IsotypeIgG2a
      Speciesbovine, human, porcine, not rat
      Positive controlHep G2 cells
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicabonate buffer, 100 µg BSA (100 µg size) or 20 µg BSA (20 µg size).
      PreservativeNone
      CommentsThis antibody recognizes an epitope on the heavy chain of mature cathepsin B and therefore detects both mature and pro-cathepsin B. Also reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesSloane, B.F. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152.
      Barret, A.J. 1987. Trends Biochem. Sci. 12, 193.
      Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 7721.
      Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol. 80, 535.
      Citation
    • A. Grimaldi, et al. (2004) Role of cathepsin B in leech wound healing. Invertebrate Survival Journal 1, 38-46.
      		•