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AM46 Anti-Caspase-8 (Ab-3) Mouse mAb (1-3)

Anti-Caspase-8, mouse mAb, clone 1-3, recognizes ~55-58 kDa latent form in non-apoptotic Jurkat cells & ~18 kDa active form in apoptotic Jurkat & HeLa cells. It is validated for Western blotting.

Anti-Caspase-8 (Ab-3) Mouse mAb (1-3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-MACH, Anti-FLICE, Anti-CAP4, Anti-CASP8, Anti-Mch5, Anti-ICE-LAP5

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~55-58 kDa latent form of caspase-8 in non-apoptotic Jurkat cells and with ~18 kDa active form in apoptotic Jurkat or HeLa cells. Additional proteins at ~42-44 kDa corresponding to the heavier processed chain may appear in some apoptotic cell lines.
      Catalogue NumberAM46
      Brand Family Calbiochem®
      SynonymsAnti-MACH, Anti-FLICE, Anti-CAP4, Anti-CASP8, Anti-Mch5, Anti-ICE-LAP5
      References
      ReferencesSmyth, L.A. and Brady, H.J.M. 2005. Hypertension 46, 100.
      Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
      Juo, P., et al. 1998. Curr. Biol. 8, 1001.
      Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
      Peter, M.E. and Krammer, P.H. 1998. Curr. Opin. Immunol. 10, 545.
      Sakamaki, K., et al. 1998. Eur. J. Biochem. 253, 399.
      Stennicke, H.R., et al. 1998. J. Biol. Chem. 273, 27084.
      Scaffifi, C., et al. 1997. J. Biol. Chem. 272, 26953.
      Stennicke, H.R. and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
      Muzio, M., et al. 1996. Cell 85, 817.
      Kischkel, F.C., et al. 1995. EMBO J. 14, 5579.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlAnti-trpE (Ab-1) Mouse mAb (I22-25) (Cat. No. OB01)
      Positive controlHeLa or Jurkat cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Not Frozen Sections
      Not Immunofluorescence
      Not Immunoprecipitation
      Not Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (not recommended)
      Application CommentsAdditional bands at ~42-44 kDa corresponding to the heavier processed chain may appear in some apoptotic cell lines. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenhuman recombinant caspase-8
      ImmunogenHuman
      Clone1-3
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AM46 0

      Documentation

      Anti-Caspase-8 (Ab-3) Mouse mAb (1-3) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Caspase-8 (Ab-3) Mouse mAb (1-3) Certificates of Analysis

      TitleLot Number
      AM46

      References

      Reference overview
      Smyth, L.A. and Brady, H.J.M. 2005. Hypertension 46, 100.
      Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
      Juo, P., et al. 1998. Curr. Biol. 8, 1001.
      Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
      Peter, M.E. and Krammer, P.H. 1998. Curr. Opin. Immunol. 10, 545.
      Sakamaki, K., et al. 1998. Eur. J. Biochem. 253, 399.
      Stennicke, H.R., et al. 1998. J. Biol. Chem. 273, 27084.
      Scaffifi, C., et al. 1997. J. Biol. Chem. 272, 26953.
      Stennicke, H.R. and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
      Muzio, M., et al. 1996. Cell 85, 817.
      Kischkel, F.C., et al. 1995. EMBO J. 14, 5579.

      Citations

      Title
    • Cecile Tetaud, et al. (2003) Two Distinct Gb3/CD77 Signaling Pathways Leading to Apoptosis are Triggered by Anti-Gb3/CD77 mAb and Verotoxin-1. Journal of Biological Chemistry 278, 45200-45208.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-August-2007 RFH
      SynonymsAnti-MACH, Anti-FLICE, Anti-CAP4, Anti-CASP8, Anti-Mch5, Anti-ICE-LAP5
      ApplicationImmunoblotting (1 µg/ml)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~56-58 kDa latent and the ~18 kDa active form of caspase-8.
      BackgroundCaspase-8 (ICE-LAP5, MACH, FLICE, CAP4) is the most proximal of the members of the apoptotic caspase cascade. Caspase-8 is directly involved in apoptosis mediated through either Fas or the TNF receptor and forms one of the key components of the transducing DISC, the CAP4 protein. Cell lines that are deficient in caspase-8 are resistant to Fas mediated apoptosis. Nine different isoforms of caspase 8 have been identified but the "a" and "b" isoforms show the highest expression at the level of protein in multiple tissues. Caspase-8 has a pH optimum of 6.8 and has a kcat/Km of 8.7 X 105 M-1 s-1. The pro-form of caspase-8 has a molecular weight of approximately 55 kDa and undergoes several processing steps to generate the active heterodimer of p18 and p10/12. In addition to activation via Fas, oligomerization of caspase-8 at the cell membrane and interaction with Granzyme B and caspase 10 will induce auto-processing and activation of the apoptotic pathway. The mouse caspase-8 gene has also been identified and is expressed in the adult as well as at high levels in the day 9.5 and day 17.5 embryo suggesting a role in development.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman recombinant caspase-8
      Clone1-3
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa or Jurkat cells
      Negative controlAnti-trpE (Ab-1) Mouse mAb (I22-25) (Cat. No. OB01)
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAdditional bands at ~42-44 kDa corresponding to the heavier processed chain may appear in some apoptotic cell lines. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesSmyth, L.A. and Brady, H.J.M. 2005. Hypertension 46, 100.
      Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
      Juo, P., et al. 1998. Curr. Biol. 8, 1001.
      Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
      Peter, M.E. and Krammer, P.H. 1998. Curr. Opin. Immunol. 10, 545.
      Sakamaki, K., et al. 1998. Eur. J. Biochem. 253, 399.
      Stennicke, H.R., et al. 1998. J. Biol. Chem. 273, 27084.
      Scaffifi, C., et al. 1997. J. Biol. Chem. 272, 26953.
      Stennicke, H.R. and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
      Muzio, M., et al. 1996. Cell 85, 817.
      Kischkel, F.C., et al. 1995. EMBO J. 14, 5579.
      Citation
    • Cecile Tetaud, et al. (2003) Two Distinct Gb3/CD77 Signaling Pathways Leading to Apoptosis are Triggered by Anti-Gb3/CD77 mAb and Verotoxin-1. Journal of Biological Chemistry 278, 45200-45208.
      		•

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies