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PC679 Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb

Anti-Caspase-3, Cleaved (Ab-2), rabbit polyclonal, recognizes the ~17 kDa cleaved fragment of caspase-3. It is validated for WB, ICC, and IHC in frozen and paraffin sections.

Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Active Caspase-3

PC679
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~17 kDa cleaved fragment of caspase-3.
      Catalogue NumberPC679
      Brand Family Calbiochem®
      SynonymsAnti-Active Caspase-3
      Application Data
      Detection of human cleaved caspase-3 by immunoblotting. Sample: Whole cell lysate from Jurkat cells treated with camptothecin (2 µM). Primary antibody: Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb (Cat. No. PC679). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2, 30% glycerol, 0.5% BSA, 5 mM EDTA.
      Positive controlA-431 cells treated with an inducer of apoptosis
      Preservative0.03% Proclin
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesFrozen Sections (10-20 µg/ml)
      Immunoblotting (0.5-1 µg/ml)
      Immunocytochemistry (10-20 µg/ml)
      Paraffin Sections (10-20 µg/ml, heat pre-treatment required)
      Application CommentsInduction of caspase-3 can occur with many different treatments, such as treatment with 0.5 µg/ml actinomycin D for 24 h. Useful for the identification of apoptotic cells in tissue sections and populations of cells. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids surrounding the Asp¹⁷⁵ cleavage site of caspase-3
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC679 0

      Documentation

      Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC679
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-January-2021 JSW
      SynonymsAnti-Active Caspase-3
      ApplicationFrozen Sections (10-20 µg/ml)
      Immunoblotting (0.5-1 µg/ml)
      Immunocytochemistry (10-20 µg/ml)
      Paraffin Sections (10-20 µg/ml, heat pre-treatment required)
      Application Data
      Detection of human cleaved caspase-3 by immunoblotting. Sample: Whole cell lysate from Jurkat cells treated with camptothecin (2 µM). Primary antibody: Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb (Cat. No. PC679). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~17 kDa cleaved fragment of caspase-3.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids surrounding the Asp¹⁷⁵ cleavage site of caspase-3
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlA-431 cells treated with an inducer of apoptosis
      FormLiquid
      FormulationIn PBS, pH 7.2, 30% glycerol, 0.5% BSA, 5 mM EDTA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative0.03% Proclin
      CommentsInduction of caspase-3 can occur with many different treatments, such as treatment with 0.5 µg/ml actinomycin D for 24 h. Useful for the identification of apoptotic cells in tissue sections and populations of cells. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling