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AB5158-50UL Anti-Calcium Channel Antibody, Voltage Gated α 1D

This Anti-Calcium Channel Antibody, Voltage Gated α 1D is validated for use in IH, IP, WB for the detection of Calcium Channel.

Anti-Calcium Channel Antibody, Voltage Gated α 1D MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters, Signaling Neuroscience Gene Symbol: CACNA1D, CCHL1A2, CACH3, Cav1.3, CACN4, CACNL1A2 UniProt Number: Q01668
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AB5158-50UL
50 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RIHC, IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5158-50UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Calcium Channel Antibody, Voltage Gated α 1D
      Alternate Names
      • L-type
      Background InformationVoltage-sensitive calcium channels (vscc) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1c gives rise to l-type calcium currents. Long-lasting (l-type) calcium channels belong to the "high-voltage activated" (hva) group. They are blocked by dihydropyridines (dhp), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-aga-IIIA). They are however insensitive to omega-conotoxin- gvia (omega-ctx-gvia) and omega-agatoxin-IVA (omega-aga-IVA) (Swiss Prot).
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 0.5-1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Calcium Channel Antibody, Voltage Gated α 1D is validated for use in IH, IP, WB for the detection of Calcium Channel.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesIn preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

      Western blot: 1:200 using ECL on rat membrane preperations. LMW form shows a 190kDa band while the HMW form shows a 210kDa band.

      Immunohistochemistry on rat brain sections.

      Dilutions should be made using a carrier protein such as BSA (1-3%).

      Optimal working dilutions and protocols must be determined by the end user.
      Biological Information
      ImmunogenPurified peptide CND1 from 859-875 of alpha 1D subunit of rat brain voltage-gated calcium channel (Accession P27732).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes all forms of a Cav1.3 (alpha 1D subunit) from the voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. Sequence conservation: hamster and mouse (16/17); human (15/17); chicken (12/17) residues identical.
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CACNA1D
      • CCHL1A2
      • CACH3
      • Cav1.3
      • CACN4
      • CACNL1A2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q01668 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the high-voltage activated (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
      SIZE: 2161 amino acids; 245141 Da
      SUBUNIT: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore- forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with RIMBP2 (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Expressed in pancreatic islets and in brain, where it has been seen in hippocampus, basal ganglia, habenula and thalamus. No expression in skeletal muscle.
      DOMAIN: SwissProt: Q01668 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
      SIMILARITY: Belongs to the calcium channel alpha-1 subunit family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB5158-50UL 04053252626005

      Documentation

      Anti-Calcium Channel Antibody, Voltage Gated α 1D MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Calcium Channel Antibody, Voltage Gated α 1D Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-a 1D SUBUNIT (Cav1.3) L-TYPE VOLTAGE GATED CALCIUM2+ CHANNEL AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2227101 2227101
      RABBIT ANTI-α 1D SUBUNIT (Cav1.3) L-TYPE VOLTAGE GATED CALCIUM2+ CHANNEL AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2391405 2391405
      RABBIT ANTI-α 1D SUBUNIT (Cav1.3) -2665007 2665007
      RABBIT ANTI-α 1D SUBUNIT (Cav1.3) L-TYPE VOLTAGE GATED CALCIUM2+ CHANNEL - 3890498 3890498

      Data Sheet

      Title
      RABBIT ANTI- 1D SUBUNIT (Cav1.3) L-TYPE VOLTAGE GATED CALCIUM2+ CHANNEL AFFINITY PURIFIED, POLYCLONAL ANTIBODY