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MABF769 Anti-CEBPB, clone 3H9 Antibody

Detect CEBPB using this Anti-CEBPB, clone 3H9 Antibody validated for use in Western Blotting and ELISA.

Anti-CEBPB, clone 3H9 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: CEBPB, LAP, TCF5, PP9092 UniProt Number: P17676
MABF769
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISAMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABF769
      Replaces04-1153
      DescriptionAnti-CEBPB, clone 3H9 Antibody
      Alternate Names
      • CCAAT/enhancer-binding protein beta
      • C/EBP beta
      • Liver activator protein
      • Nuclear factor NF-IL6
      • Transcription factor 5
      • TCF-5
      • CEBPB
      Background InformationThe transcription factor CCAAT/Enhancer Binding Protein beta (C/EBPβ, also known as NFIL6) is a highly conserved B-Zip family member. C/EBPβ regulates multiple genes involved in hepatic function, immune function and adipogenesis, and its functions are generally distinct from those of other C/EBP family members (alpha, gamma, delta, epsilon). C/EBPβ is present in cells as three alternate translation products: two transcription activating forms known as LAP1 and LAP2 (~35 and 32 kDa in rodents, ~43 kDa and 40 kDa in humans) and a truncated inhibitory form known as LIP (~20 kDa) which lacks the N-terminal transcription activation domain. C/EBP β is phosphorylated at multiple sites which are determinants of some of its activities (e.g. activation of c-fos, adipogenesis). A recent report shows that it is acetylated at Lys215/216, and others report that it can be sumoylated. C/EBPβ is also acetylated at Lys39 in the transcription activation domain, and mutation of Lys39 impairs its ability to activate transcription of multiple genes, including several involved in adipogenesis.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationDetect CEBPB using this Anti-CEBPB, clone 3H9 Antibody validated for use in Western Blotting and ELISA.
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
      Biological Information
      ImmunogenPurified recombinant fragment of human CEBPB expressed in E. Coli.
      Clone3H9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • CEBPB
      • LAP
      • TCF5
      • PP9092
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~42 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in CEBPB recombinant protein.

      Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected CEBPB in recombinant protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF769 04055977171846