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ABS2270-25UL Anti-CDK11

Anti-CDK11, Cat. No. ABS2270, is a rabbit polyclonal antibody that detects Cyclin-dependent kinase 11 (CDK11) and is tested for use in ChIP-seq and Western Blotting

ABS2270-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2270-25UL
      DescriptionAnti-CDK11
      Alternate Names
      • Cyclin-dependent kinase 11A
      • EC:2.7.11.22
      • Cell division cycle 2-like protein kinase 2
      • Cell division protein kinase 11A
      • Galactosyltransferase-associated protein kinase p58/GTA
      • PITSLRE serine/threonine-protein kinase CDC2L
      Background InformationCyclin-dependent kinase 11A (UniProt: Q9UQ88; also known as EC:2.7.11.22, Cell division cycle 2-like protein kinase 2, Cell division protein kinase 11A, Galactosyltransferase-associated protein kinase p58/GTA, PITSLRE serine/threonine-protein kinase CDC2L2) is encoded by the CDK11A (also known as CDC2L2, CDC2L3, PITSLREB) gene (Gene ID: 728642) in human. CDK11 is a ubiquitously expressed protein that plays multiple roles in cell cycle progression, cytokinesis, and apoptosis. Eight different isoforms of CDK11 have been described that are produced by alternative splicing and alternative initiation. The p110 isoform (canonical) is suggested to be involved in pre-mRNA splicing, potentially by phosphorylating the splicing protein SFRS7. During apoptosis, it is induced by Fas or tumor necrosis factor. The p110 isoform is cleaved by caspases to produce p110C form that contains the C-terminal kinase domain and binds to the serine/threonine kinase PAK1. CDK11 is reported to associate with SF3B1 and phosphorylates threonine residues at its N terminus during spliceosome activation. This phosphorylation event is shown to be essential for the association between SF3B1 and U5 and U6 snRNAs in the activated spliceosome, termed the Bact complex. CDK11 is overexpressed in several tumor types and is a predictor of poor clinical outcome. (Ref.: Blazek, D. (2023). Clin. Transl Med. 13(3); e1201; Hluchý, M., et al. (2022). Nature. 609(7928); 829-834).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody in serum without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CDK11, Cat. No. ABS2270, is a rabbit polyclonal antibody that detects Cyclin-dependent kinase 11 (CDK11) and is tested for use in ChIP-seq and Western Blotting
      Key Applications
      • Western Blotting
      • ChIP-seq
      Application NotesWestern Blotting Analysis: A representative lot detected Cyclin-dependent kinase 11 in Western Blotting applications (Hluchy, M., et. al. (2022). Nature. 609(7928):829-834).

      ChIP-seq Anaylysis: A representative lot detected Cyclin-dependent kinase 11 in ChIP-seq applications (Hluchy, M., et. al. (2022). Nature. 609(7928):829-834).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to the first 226 amino acids from the N-terminal region of human Cyclin-dependent kinase 11A (CDK11A).
      EpitopeN-terminal
      Clonerabbit #5
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Cyclin-dependent kinase 11A (CDK11) and targets an epitope within the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDK11A
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~95 kDa observed; 91.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HCT116 Cell Lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CDK11 in HCT116 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2270-25UL 04065270845436