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217580 Anti-CD8 Mouse mAb (UCHT4)

Anti-CD8 Mouse mAb (UCHT4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
217580
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~33 kDa CD8 protein on human T lymphocytes.
      Catalogue Number217580
      Brand Family Calbiochem®
      References
      ReferencesFischer, A., et al. 1983. Immunology 48, 177.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM potassium chloride, 50 mM sodium phosphate, pH 7.5.
      Preservative0.5 mg/ml gentamycin sulfate
      Applications
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoprecipitation
      Application NotesFlow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      Application CommentsFACS was performed on 5x105 ficoll prepared human peripheral white blood cells and analyzed by flow cytometry. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman thymocytes or Sezary T cells
      ImmunogenHuman
      CloneUCHT4
      HostMouse
      Purity>95% immunoglobulin by SDS-PAGE
      IsotypeIgG2a
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsIn cases where freezing is desired, avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      217580 0

      Documentation

      Anti-CD8 Mouse mAb (UCHT4) Certificates of Analysis

      TitleLot Number
      217580

      References

      Reference overview
      Fischer, A., et al. 1983. Immunology 48, 177.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      ApplicationFlow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~33 kDa CD8 α-chain protein.
      BackgroundHuman CD8 is a heterodimeric protein consisting of an α and β chain, each about 33 kDa. CD8 is expressed on most thymocytes and on about one third of peripheral blood T cells. CD8 is a co-receptor involved in antigen recognition and binds to the α2 and α3 domain of MHC Class molecules.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman thymocytes or Sezary T cells
      CloneUCHT4
      IsotypeIgG2a
      Specieshuman
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM potassium chloride, 50 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity>95% immunoglobulin by SDS-PAGE
      Preservative0.5 mg/ml gentamycin sulfate
      CommentsFACS was performed on 5x105 ficoll prepared human peripheral white blood cells and analyzed by flow cytometry. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsIn cases where freezing is desired, avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesFischer, A., et al. 1983. Immunology 48, 177.