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ST1515 Anti-CAMK1 Mouse mAb (3G1)

ST1515
  
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      Overview

      Replacement Information

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~38 kDa CAMK1 protein in HL-60 cells.
      Catalogue NumberST1515
      Brand Family Calbiochem®
      SynonymsAnti-Calcium/Calmodulin Dependent Protein Kinase 1
      Application Data
      Detection of human CAMK1 by immunoblotting. Sample: Whole cell lysate from HL-60 cells. Primary antibody: Anti-CAMK1 Mouse mAb (3G1) (Cat. No. ST1515) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlHL-60 cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids partial 271-371 of human CAMK1, expressed as a GST fusion protein
      ImmunogenNA
      Clone3G1
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1515 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Calcium/Calmodulin Dependent Protein Kinase 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human CAMK1 by immunoblotting. Sample: Whole cell lysate from HL-60 cells. Primary antibody: Anti-CAMK1 Mouse mAb (3G1) (Cat. No. ST1515) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~38 kDa CAMK1 protein.
      BackgroundCalcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
      HostMouse
      Immunogen speciesNA
      ImmunogenA recombinant polypeptide corresponding to amino acids partial 271-371 of human CAMK1, expressed as a GST fusion protein
      Clone3G1
      IsotypeIgG₁
      Specieshuman
      Positive controlHL-60 cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review