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AB17003 Anti-Bim Antibody, internal epitope, pan-Bim isoforms

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AB17003
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB17003
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Bim Antibody, internal epitope, pan-Bim isoforms
      Alternate Names
      • BOD
      Product Information
      FormatPurified
      Control
      • Skin/Skin Tumor
      PresentationImmunoaffinity Purified immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Bim Antibody, internal epitope, pan-Bim isoforms is validated for use in WB for the detection of Bim.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:1000-1:2000

      Human K562 cell lysate can be used as a positive control and a 23 kDa band can be detected.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 22-40 of human origin (O'Conneor et al. 1998). The sequence is identical to that of mouse and is different by one amino acid from that of rat.
      Epitopeinternal epitope, pan-Bim isoforms
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes pan-Bim isoforms.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFunction: Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
      Subunit structure: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK By similarity.
      Subcellular location: Endomembrane system; Peripheral membrane protein By similarity. Note: Associated with intracytoplasmic membranes By similarity.
      Isoform BimEL: Mitochondrion
      Isoform BimL: Mitochondrion
      Isoform BimS: Mitochondrion
      Isoform Bim-alpha1: Mitochondrion
      Tissue specificity: Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. Ref.3
      Domain: The BH3 motif is required for Bcl-2 binding and cytotoxicity.
      Sequence similarities: Belongs to the Bcl-2 family.
      Molecular Weight23 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe undiluted antibody solution is stable for approximately 6 months when stored 2–8°C.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      AB17003 04053252405907