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ST1088 Anti-Akt, PH Domain Mouse mAb (SKB1)

Anti-Akt, PH Domain, mouse monoclonal, clone SKB1, recognizes the ~60 kDa Akt protein in A431 cells. It is validated for flow cytometry, Western blotting, and immunoprecipitation.

Anti-Akt, PH Domain Mouse mAb (SKB1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-PKB, Pleckstrin Homology Domain

ST1088
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

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      Description
      OverviewRecognizes the ~60 kDa Akt protein in A431 cells. Useful for the concentration of Akt in samples by immunoprecipitation prior to use in the K-LISA™ Akt Activity Kit (Cat. No. CBA019).
    • Antibody Target Gene Symbol: AKT1
    • Target Synonym: AKT, MGC99656, PKB, PKB-ALPHA, PKB/AKT, PRKBA, Protein kinase B, RAC, RAC-ALPHA, Thymoma viral proto-oncogene 1
    • Entrez Gene Name: v-akt murine thymoma viral oncogene homolog 1
    • Hu Entrez ID: 207 (Related Antibodies: 530311, PK1203, PC510 and 124001)
    • Mu Entrez ID: 11651
    • Rat Entrez ID: 24185
      		•
      Catalogue NumberST1088
      Brand Family Calbiochem®
      SynonymsAnti-PKB, Pleckstrin Homology Domain
      Application Data
      Detection of human Akt by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-Akt, PH Domain Mouse mAb (SKB1) (Cat. No. ST1088) (0.5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesBellacosa, A., et al. 1998. Oncogene 17, 313.
      Product Information
      FormLiquid
      Formulation100 µg antibody in 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4 and 100 µg control lysate.
      Positive controlA431 cells (included)
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Bourguignon, L.Y.W., et al. 2003. J. Biol. Chem. 278, 29420. Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958. Immunoprecipitation, Active Enzyme Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958.
      Key Applications Flow Cytometry
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesFlow Cytometry (see comments)
      Immunoblotting (0.5-2 µg/ml, see application references)
      Immunoprecipitation (4 µg/0.5 mg total protein; see comments and see application references)
      Application CommentsThis antibody will immunoprecipitate active Akt from cell lysates. Useful for immunoprecipitation of Akt prior to activity assay using the K-LISA™ Akt Activity Kit (Cat. No. CBA019). This antibody has also been reported to work for flow cytometry applications. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 1-149 of human Akt 1 fused to GST
      ImmunogenHuman
      CloneSKB1
      HostMouse
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1088-1SET 04055977224412

      Documentation

      Anti-Akt, PH Domain Mouse mAb (SKB1) Certificates of Analysis

      TitleLot Number
      ST1088

      References

      Reference overview
      Bellacosa, A., et al. 1998. Oncogene 17, 313.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-August-2008 JSW
      SynonymsAnti-PKB, Pleckstrin Homology Domain
      ApplicationFlow Cytometry (see comments)
      Immunoblotting (0.5-2 µg/ml, see application references)
      Immunoprecipitation (4 µg/0.5 mg total protein; see comments and see application references)
      Application Data
      Detection of human Akt by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-Akt, PH Domain Mouse mAb (SKB1) (Cat. No. ST1088) (0.5 µg/ml). Detection: chemiluminescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes ~50-60 kDa Akt1 and Akt2 proteins. Supplied with control lysate derived from unstimulated A431 cells.
      BackgroundAkt (Accession No. NM_005163) is primarily a cytosolic enzyme that acts as a downstream effector of phosphatidylinositol 3-kinase (PI-3K) activation. Akt has been shown to be activated by a variety of stimuli, including hematopoetic cytokines, various chemokines, heat shock, hypoxia,hyperosmolarity, integrins, nerve growth factor (NGF), platelet- derived growth factor (PDGF), and T cell receptor signaling. Suggested to play a role in regulating the balance between cellular mitogenesis and apoptosis. Akt has been shown to be constitutively active in some human cancers.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 1-149 of human Akt 1 fused to GST
      CloneSKB1
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlA431 cells (included)
      FormLiquid
      Formulation100 µg antibody in 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4 and 100 µg control lysate.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody will immunoprecipitate active Akt from cell lysates. Useful for immunoprecipitation of Akt prior to activity assay using the K-LISA™ Akt Activity Kit (Cat. No. CBA019). This antibody has also been reported to work for flow cytometry applications. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBellacosa, A., et al. 1998. Oncogene 17, 313.
      Application referencesImmunoblotting Bourguignon, L.Y.W., et al. 2003. J. Biol. Chem. 278, 29420. Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958. Immunoprecipitation, Active Enzyme Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958.