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MABS2152-25UG Anti-ATP8 Antibody, clone 1G3-1H11-1C3

Anti-ATP8, clone 1G3-1H11-1C3, Cat. No. MABS2152, is a mouse monoclonal antibody that detects ATP synthase protein 8 (ATP8) and has been tested for use in Western Blotting.

MABS2152-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2152-25UG
      DescriptionAnti-ATP8 Antibody, clone 1G3-1H11-1C3
      Alternate Names
      • ATP synthase protein 8
      • A6L
      • F-ATPase subunit 8
      Background InformationATP synthase protein 8 (UniProt: P03928; also known as A6L, F-ATPase subunit 8) is encoded by the MT-ATP8 (also known as ATP8, ATPASE8, MTATP8) gene (Gene ID: 4509) in human. ATP8 is a single-pass inner mitochondrial membrane protein whose transmembrane domain is localized to amino acids 8-24. It is an F-type ATPase that produced ATP from ADP in the presence of a protein gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is responsible for the final step in oxidative phosphorylation and in the electron transport chain.
      F-type ATPases consist of tan F1-containing extramembraneous catalytic core and a F0-containing the membrane proton channel. They are linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Mutations in MT-ATP8 gene is reported to cause the deficiency of mitochondrial complex V that leads to heterogeneous clinical manifestations, including neuropathy, ataxia, and hypertrophic cardiomyopathy. (Ref.: Jonckheere, AI., et al., (2008). J. Med. Genet. 45(3); 129-133).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 150 mM NaCl, 15 mM HEPES, pH 7.5 with 0.02% sodium azide.
      Applications
      ApplicationAnti-ATP8, clone 1G3-1H11-1C3, Cat. No. MABS2152, is a mouse monoclonal antibody that detects ATP synthase protein 8 (ATP8) and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenA synthetic peptide corresponding to the N-terminus of human mitochondrial ATP8.
      Clone1G3-1H11-1C3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1G3-1H11-1C3 is a mouse monoclonal antibody that specifically detects human mitochondrial ATPsynthase protein 8 (ATP8).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • MT-ATP8:ATP8
      • ATPASE8
      • MTATP8
      Purification MethodProtein L
      UniProt Number
      Molecular Weight~9.8 kDa observed; 8.0 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mitochondria from neonatal human dermal fibroblasts (HdFn).

      Western Blotting Analysis: 1 µg/mL of this antibody detected ATP synthase protein 8 (ATP8) in mitochondria from neonatal human dermal fibroblasts (HdFn), but not in mitochondria depleted of MtDNA (Rho0) following treatment with ethidium bromide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2152-25UG 04061841030423