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MABN2302-100UL Anti-ARHGAP11B Antibody, clone 3758-A37-5

Anti-ARHGAP11B, clone 3758-A37-5, Cat. No. MABN2302, is a mouse monoclonal antibody that detects ARHGAP11B and is tested for use in Immunofluorescence and Western Blotting.

MABN2302-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2302-100UL
      DescriptionAnti-ARHGAP11B Antibody, clone 3758-A37-5
      Alternate Names
      • Inactive Rho GTPase-activating protein 11B
      • Rho-type GTPase-activating protein 11B
      Background InformationInactive Rho GTPase-activating protein 11B (UniProt: Q3KRB8; also known as Rho-type GTPase-activating protein 11B) is encoded by the ARHGAP11B (also known as FAM7B1) gene (Gene ID: 89839) in human. ARHGAP11B is a mitochondrial matrix protein that is specifically expressed in radial glia cells of cortical neural stem and progenitor cells during neocortex development. It is preferentially expressed in apical and basal radial glia and is not detected in cortical neurons and cortical plate. It promotes amplification of basal progenitors in the subventricular zone, producing more neurons during fetal corticogenesis, thereby playing a key role in neocortex expansion. It promotes basal progenitor cell proliferation by inhibiting the mitochondrial permeability transition pore (mPTP). It delays the opening of the mPTP via interaction with ADP:ATP translocase, thereby increasing mitochondrial Ca2+ concentration and inducing glutamine catabolism, which is required for basal progenitor proliferation. ARHGAP11B does not exhibit GTPase activator activity and this lack of GTPase activator activity is essential for amplification of basal progenitors during neocortex development. (Ref.: Namba, T., et al. (2020). Neuron. 105(5); 867-881; Florio, M., et al. (2015). Science. 347(6229); 1465-1470).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-ARHGAP11B, clone 3758-A37-5, Cat. No. MABN2302, is a mouse monoclonal antibody that detects ARHGAP11B and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested applications

      Immunofluorescence Analysis: A representative lot detected ARHGAP11B in Immunofluorescence application (Namba, T., et al. (2020). Neuron. 105(5):867-881.e9; Heide, M., et al. (2020). Science. 369(6503):546-550).

      Western Blotting Analysis: A representative lot detected ARHGAP11B in Western Blotting applications (Namba, T., et al. (2020). Neuron. 105(5):867-881.e9).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Rho-type GTPase-activating protein 11B (ARHGAP11B).
      EpitopeC-terminal
      Clone3758-A37-5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3758-A37-5 is a mouse monoclonal antibody that detects Rho-type GTPase-activating protein 11B (ARHGAP11B). It targets an epitope within 21 amino acids from the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ARHGAP11B
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~30 kDa observed; 30.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from COS-7 cells transfected with human ARHGAP11B.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected ARHGAP11B in lysate from COS-7 cells transfected with human ARHGAP11B, but not in lysate from wild-type COS-7 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2302-100UL 04065270172631