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NA81 Anti-5-Methylcytosine Mouse mAb (162 33 D3)

This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.

Anti-5-Methylcytosine Mouse mAb (162 33 D3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-5-mc, Anti-5-MeCyd

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NA81
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Pricing & Availability

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NA81-50UG
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      Description
      OverviewRecognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.

      Specific
      • Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
      • Flow Cytometry
      • Frozen Sections
      • Immunoblotting
      • Immunofluorescence
      • Paraffin Sections
      Reliable
      • Generates reproducible results.
      • Clone 162 33 D3 has been used in over 50 publications.

      Catalogue NumberNA81
      Brand Family Calbiochem®
      SynonymsAnti-5-mc, Anti-5-MeCyd
      Application Data
      Detection of 5-methylcytosine by immunohistochemistry. Sample: Normal colon (left) and colon adenocarcinoma (right). Anti-5-Methylcytosine Mouse mAb (162 33 D3) (Cat. No. NA81). Detection: DAB

      Chromosome methylation patterns of mouse embryos at the one-cell stage. Methylated sites were revealed by indirect immunofluorescence labeling with Anti-5-methylcytosine.
      References
      ReferencesEhrlich, M. 2002. Oncogene 21, 5400. (Review)
      Widschwendter, M. and Jones, P.A. 2002. Oncogene 21, 5462. (Review)
      Barton, S.C., et al. 2001. Hum. Mol. Genet. 10, 2983.
      Piyathilake, C.J., et al. 2001. Hum. Pathol. 32, 856.
      Taddei, A., et al. 2001. Nat. Cell Biol. 3, 114.
      Piyathilake, C.J., et al. 2000. Biotech. Histochem. 75, 251.
      De Capoa, A., et al. 1999. FASEB J. 13, 89.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.4.
      Positive controlNIH3T3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections Kang, J.S., et al. 2006. Cancer Sci. 97, 453.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Paraffin Sections
      Radioimmunoassay
      Southwestern Blot
      Application NotesELISA (0.1 µg/ml)
      Flow Cytometry (1 µg/ml)
      Frozen Sections (5-10 µg/ml)
      Immunoblotting (5 µg/ml)
      Immunofluorescence (1 µg/ml)
      Paraffin Sections (5-10 µg/ml)
      Radioimmunoassay (0.1 µg/ml)
      Southwestern Blot (5 µg/ml)
      Application CommentsClone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.
      Biological Information
      Immunogen5-methylcytosine conjugated to ovalbumin
      Clone162 33 D3
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NA81-50UG 04055977209792

      Documentation

      Anti-5-Methylcytosine Mouse mAb (162 33 D3) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-5-Methylcytosine Mouse mAb (162 33 D3) Certificates of Analysis

      TitleLot Number
      NA81

      References

      Reference overview
      Ehrlich, M. 2002. Oncogene 21, 5400. (Review)
      Widschwendter, M. and Jones, P.A. 2002. Oncogene 21, 5462. (Review)
      Barton, S.C., et al. 2001. Hum. Mol. Genet. 10, 2983.
      Piyathilake, C.J., et al. 2001. Hum. Pathol. 32, 856.
      Taddei, A., et al. 2001. Nat. Cell Biol. 3, 114.
      Piyathilake, C.J., et al. 2000. Biotech. Histochem. 75, 251.
      De Capoa, A., et al. 1999. FASEB J. 13, 89.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-August-2008 JSW
      SynonymsAnti-5-mc, Anti-5-MeCyd
      ApplicationELISA (0.1 µg/ml)
      Flow Cytometry (1 µg/ml)
      Frozen Sections (5-10 µg/ml)
      Immunoblotting (5 µg/ml)
      Immunofluorescence (1 µg/ml)
      Paraffin Sections (5-10 µg/ml)
      Radioimmunoassay (0.1 µg/ml)
      Southwestern Blot (5 µg/ml)
      Application Data
      Detection of 5-methylcytosine by immunohistochemistry. Sample: Normal colon (left) and colon adenocarcinoma (right). Anti-5-Methylcytosine Mouse mAb (162 33 D3) (Cat. No. NA81). Detection: DAB

      Chromosome methylation patterns of mouse embryos at the one-cell stage. Methylated sites were revealed by indirect immunofluorescence labeling with Anti-5-methylcytosine.
      DescriptionImmunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine.
      BackgroundDNA methylation plays an important role in gene regulation. Methylation of gene-promoter regions leads to loss of function while DNA demethylation can lead to gain of function. Alterations in DNA methylation may play an important role in carcinogenesis. DNA methylation varies according to tissue type. In some cancers types such as Wilms tumor and colon adenocarcinoma have a decrease in global DNA methylation. In contrast other cancers such as breast cancer have DNA hypermethylation. Changes in global DNA methylation status may be useful early markers for the detection of premalignant lesions.
      HostMouse
      Immunogen5-methylcytosine conjugated to ovalbumin
      Clone162 33 D3
      IsotypeIgG₁
      Speciesa broad range of species
      Positive controlNIH3T3 cells
      FormLiquid
      FormulationIn PBS, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsClone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesEhrlich, M. 2002. Oncogene 21, 5400. (Review)
      Widschwendter, M. and Jones, P.A. 2002. Oncogene 21, 5462. (Review)
      Barton, S.C., et al. 2001. Hum. Mol. Genet. 10, 2983.
      Piyathilake, C.J., et al. 2001. Hum. Pathol. 32, 856.
      Taddei, A., et al. 2001. Nat. Cell Biol. 3, 114.
      Piyathilake, C.J., et al. 2000. Biotech. Histochem. 75, 251.
      De Capoa, A., et al. 1999. FASEB J. 13, 89.
      Application referencesParaffin Sections Kang, J.S., et al. 2006. Cancer Sci. 97, 453.