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616385 α-Toxin, Staphylococcus aureus - CAS 12616-52-3 - Calbiochem

Alpha Toxin, CAS 12616-52-3, is a major cytotoxin isolated and purified from the Wood 46 strain of Staphylococcus aureus. At low concentration it binds to cell surface receptors and forms heptameric pores.

α-Toxin, Staphylococcus aureus - CAS 12616-52-3 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information

      Key Spec Table

      CAS #
      12616-52-3
      Description
      Overview

      This product has been discontinued.



      A monomer isolated from the Wood 46 strain of Staphylococcus aureus and purified by a modification of the methods of Harshman, et al., and Hohman. Stimulates prostaglandin synthesis and phosphorylation of myelin basic protein. Causes internucleosomal DNA degradation in T lymphocytes by forming small transmembrane pores.<.p>
      Hemolytic activity: ≥30 units/µg.
      Catalogue Number616385
      Brand Family Calbiochem®
      References
      ReferencesJonas, D., et al. 1994. Infect. Immun. 62, 1304.
      Cescatti, L., et al. 1991. J. Membr. Biol. 119, 53.
      Harshman. S., et al. 1988. Methods Enzymol. 165, 3.
      Hohman, R.J. 1988. Proc. Natl. Acad. Sci. USA 85, 1624.
      Product Information
      CAS number12616-52-3
      Unit of DefinitionOne unit is defined as the dilution of α-toxin that mediates 50% lysis of a 5% suspension of rabbit red cells.
      ATP CompetitiveN
      DeclarationNot available for sale outside of the United States.
      FormSolid
      FormulationLyophilized from 10 mM sodium phosphate buffer, pH 7.2.
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetStimulates prostaglandin synthesis and phosphorylation of myelin basic protein.
      PuritySingle band by SDS-PAGE
      SourceA single polypeptide chain protein isolated from the Wood 46 strain of Staphylococcus aureus and purified by a modification of the methods of Harshman, S., et al. (1988) and Hohman R.J. (1988).
      Specific Activity≥30 units/µg protein
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSWI0195720
      Safety Information
      R PhraseR: 26/27/28

      Very toxic by inhalation, in contact with skin and if swallowed.
      S PhraseS: 36/37/39-45

      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Highly Toxic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for one week at 4°C.
      End use certificateY
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      616385 0

      Documentation

      α-Toxin, Staphylococcus aureus - CAS 12616-52-3 - Calbiochem MSDS

      Title

      Safety Data Sheet (SDS) 

      α-Toxin, Staphylococcus aureus - CAS 12616-52-3 - Calbiochem Certificates of Analysis

      TitleLot Number
      616385

      References

      Reference overview
      Jonas, D., et al. 1994. Infect. Immun. 62, 1304.
      Cescatti, L., et al. 1991. J. Membr. Biol. 119, 53.
      Harshman. S., et al. 1988. Methods Enzymol. 165, 3.
      Hohman, R.J. 1988. Proc. Natl. Acad. Sci. USA 85, 1624.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 RFH
      Descriptionα-Toxin causes internucleosomal DNA degradation in T lymphocytes by forming small transmembrane pores. Many cell types are sensitive to cytolysis by α-toxin. Under certain conditions, nucleated cells can be treated with α-toxin without causing lysis. Under controlled conditions, α-toxin monomers bind to the cell membrane and form a hexamer, which functions as a hydrophilic pore with a 1-3 nm diameter. The cell becomes permeable to small molecules, while large molecules, such as enzymes or carbohydrates are excluded. The molecular weight cut-off of material passing through the pore is approximately 2-4 kDa. The pore is somewhat ion selective. Other agents commonly used for permeabilizing cells form large lesions in the membrane, making them permeable to macromolecules. α-Toxin has been utilized to introduce ions, substrates, inhibitors, nucleotides, and other pharmacological agents into cells, thereby regulating cell physiology or function. For example, the arachidonic acid cascade (and subsequent production of prostaglandin I2) is triggered by α-toxin and PMNs in endothelial cells when pores are formed that allow the passive influx of Ca2+. Stimulates prostaglandin synthesis and enhances phosphorylation of myelin basic protein.
      FormSolid
      FormulationLyophilized from 10 mM sodium phosphate buffer, pH 7.2.
      SourceA single polypeptide chain protein isolated from the Wood 46 strain of Staphylococcus aureus and purified by a modification of the methods of Harshman, S., et al. (1988) and Hohman R.J. (1988).
      CAS number12616-52-3
      RTECSWI0195720
      PuritySingle band by SDS-PAGE
      Specific activity≥30 units/µg protein
      Unit definitionOne unit is defined as the dilution of α-toxin that mediates 50% lysis of a 5% suspension of rabbit red cells.
      SolubilityReconstitute with 500 µl of sterile distilled H₂O
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for one week at 4°C.
      Toxicity Highly Toxic
      ReferencesJonas, D., et al. 1994. Infect. Immun. 62, 1304.
      Cescatti, L., et al. 1991. J. Membr. Biol. 119, 53.
      Harshman. S., et al. 1988. Methods Enzymol. 165, 3.
      Hohman, R.J. 1988. Proc. Natl. Acad. Sci. USA 85, 1624.