western blotting
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Sample Preparation and Gel Electrophoresis | Life Science Research | Merck
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions. -
Preparing cell lysates | Life Science Research | Merck
This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total protein concentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the sensitivity and reproducibility of the Western blot. -
Transfer Membranes | Life Science Research | Merck
Our transfer membranes include PVDF membranes optimized for different protein blotting applications, blotting sandwiches, and blotting filter paper. -
SNAP i.d.® 2.0 IHC System Videos | Life Science Research | Merck
SNAP i.d.® Protein Detection System for Immunohistochemistry (IHC) is the latest capability of the SNAP i.d.® 2.0 System. It allows you to process up to 24 slides at a time with reduced handling time and variability. The system is compatible with standard IHC slides and protocols and works with diverse tissue preparations including formalin-fixed and fresh frozen samples. Watch this video for an introduction to the innovative, vacuum driven system for IHC. -
Immobilon®-P PVDF Membrane | Life Science Research | Merck
Immobilon-P PVDF membrane is the most commonly used PVDF transfer membrane for Western blotting. Available in sheets, rolls, and pre-cut sandwiches. -
SNAP i.d.® 2.0 System Videos | Life Science Research | Merck
Watch these SNAP id 2.0 videos to learn more about the system components, its operation, and how it will speed up your workflow without sacrificing results. -
Immobilon®-FL Membrane | Life Science Research | Merck
The Immobilon-FL membrane is the first transfer membrane optimized for fluorescence applications. It has low background for sensitivity in all detection protocols. -
SNAP i.d.® 2.0 Protein Detection System | Life Science Research | Merck
We designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry (IHC) experiments. -
Immobilon®-PSQ Membrane | Life Science Research | Merck
Our Immobilon-PSQ membrane has higher protein adsorption and sequencing yields than other membranes, a 0.2 µm pore size and large internal structure.