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freezing+media


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  • A simple and efficient cryopreservation method for feeder-free dissociated human induced pluripotent stem cells and human embryonic stem cells. 19602515

    An essential prerequisite for the future widespread application of human induced pluripotent (hiPSCs) and embryonic stem cells (hESCs) is the development of efficient cryopreservation methods to facilitate their storage and transportation.We developed a simple and effective freezing/thawing method of single dissociated hESCs and...
    Dokumentumtípus:
    Reference
    Katalógusszám:
    Multiple
    Katalógusszám:
    Multiple

  • Dosage and cell line dependent inhibitory effect of bFGF supplement in human pluripotent stem cell culture on inactivated human mesenchymal stem cells. 24465853

    Many different culture systems have been developed for expanding human pluripotent stem cells (hESCs and hiPSCs). In general, 4-10 ng/ml of bFGF is supplemented in culture media in feeder-dependent systems regardless of feeder cell types, whereas in feeder-free systems, up to 100 ng/ml of bFGF is required for maintaining long-te...
    Dokumentumtípus:
    Reference
    Katalógusszám:
    MAB4304
    Katalógusszám:
    Anti-Stage-Specific Embryonic Antigen-4 Antibody, clone MC-813-70

  • WEHI 164 subclone 13 assay for TNF: sensitivity, specificity, and reliability. 2110931

    Tumor necrosis factor alpha (TNF) is a peptide monokine involved in a number of immune reactions. To further understand the role of TNF in disease states it is critical to have an inexpensive, yet sensitive and specific assay. Additionally, the effects of prostaglandin E2 (PGE2), dexamethasone (dex), and cyclosporine A (CsA) on...
    Dokumentumtípus:
    Reference
    Katalógusszám:
    01-164

  • Derivation of mouse embryonic stem cells 17487198

    Here we describe a simple and efficient protocol for derivation of germline chimera-competent mouse embryonic stem cells (mESCs) from embryonic day 3.5 (E3.5) blastocysts. The protocol involves the use of early-passage mouse embryonic fibroblast feeders (MEF) and the alternation of fetal bovine serum- and serum replacement (SR)-...
    Dokumentumtípus:
    Reference
    Katalógusszám:
    Multiple
    Katalógusszám:
    Multiple

  • Derivation of a new human embryonic stem cell line, Endeavour-2, and its characterization. 20178001

    Here, we describe the derivation of a novel human embryonic stem cell (hESC) line, Endeavour-2 (E-2), propagated on human fetal fibroblasts (HFF) in a serum-replacement media. The inner cell mass (ICM) was manually dissected from the blastocyst without using immunodissection and, therefore, antibodies from animal sources. A tota...
    Dokumentumtípus:
    Reference
    Katalógusszám:
    MAB1982
    Katalógusszám:
    Anti-Integrin α6 Antibody, clone MA6
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