71297 rLysozyme™ Solution, Veggie Grade

rLysozyme Solution, Veggie™ Grade is a special grade of rLysozyme prepared using certified animal-free or disease-free reagents. All of the steps in the preparation of the recombinant enzyme are carried out using reagents of nonanimal origin, with the exception of the IPTG used to induce protein expression. IPTG is chemically synthesized by a stringent process from D-galactose isolated from lactose, a milk sugar. The lactose is derived from certified disease-free cows. rLysozyme Solution, Veggie Grade has the same stability and specific activity as the rLysozyme Solution and requires no change to the protocol.

With its high specific activity (1700 KU/mg), only a small amount of rLysozyme Solution, Veggie Grade is required to break the cell wall of E. coli. Use the enzyme at 3-5 KU/g cells to enhance protein recovery from cells treated with BugBuster or BugBuster HT protein extraction reagents or with PopCulture Reagent. Or, treat thawed cell pellets with only the enzyme using 45-60 KU/g cells. Optimal protein purification is achieved by treating the cell lysate with Benzonase® Nuclease, which efficiently reduces extract viscosity by degrading the nucleic acids liberated as cells lyse. rLysozyme Solution, Veggie Grade is supplied as a ready-to-use solution at a concentration of 30 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON X-100, pH 7.5. The solution is stable at -20°C.