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69658 pLysE DNA

pLysE DNA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69658
  
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Áttekintés

Replacement Information
Description
Overview

This product has been discontinued.





pLysS and pLysE are 4886bp plasmids constructed by insertion of the T7 lysozyme gene into the BamH I site of pACYC184 (1). These plasmids are not cloning vectors; they are used in λDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase. The two plasmids differ only by the orientation of the T7 lysozyme gene. In pLysS the T7 lysozyme coding sequence is in the antisense orientation relative to the tet promoter, so only a small amount of T7 lysozyme is produced. In pLysE large amounts of T7 lysozyme are produced from the tet promoter. The construct also contains the weak T7 φ3.8 promoter immediately following the lysozyme gene. The p15A origin of replication is compatible with those found in pBR322- and pUC-derived plasmids.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
Catalogue Number69658
Brand Family Novagen®
References
References1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
69658 0

Documentation

pLysE DNA MSDS

Title

Safety Data Sheet (SDS) 

pLysE DNA Certificates of Analysis

TitleLot Number
69658

References

Hivatkozások áttekintése
1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.

Citations

Titulus
  • Mark S. McClain and Timothy L. Cover. (2007) Functional analysis of neutralizing antibodies against Clostridium perfringens epsilon-toxin. Infection and Immunity 75, 1785-1793.
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    Vector Map

    Title
    TB107 pLysS and pLysE Plasmid Map

    Vector Sequence

    Title
    pLysE Sequence