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71328 pET Expression System 45b

pET vectors plus host strains with induction control

pET Expression System 45b MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71328
  
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Áttekintés

Replacement Information
Description
Overview

This product has been discontinued.





pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock

Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid

These components are sufficient for up to 10 transformations in each host.

Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

pET Expression System 45b
The pET Expression System 4b5 contains 10 µg of pET-45b(+) DNA. pET-45b carries an N-terminal His•Tag® coding sequence that is cleavable with enterokinase. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB396.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number71328
Brand Family Novagen®
References
Product Information
•10 µgpET Vector DNA
•0.2 mlHost bacterial strains BL21, BL21(DE3) and BL21(DE3) pLysS Glycerol Stocks
•0.2 mlInduction Control
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
71328 0

Documentation

pET Expression System 45b Certificates of Analysis

TitleLot Number
71328

Citations

Titulus
  • Robert T. Glover, et al. (2007) The two-component regulatory system senX3-regX3 regulates phosphate-dependent gene expression in Mycobacterium smegmatis. Journal of Bacteriology 189, 5495-5503.
  • Guifeng Sun, et al. (2007) Structural and functional analyses of the major outer membrane protein of Chlamydia trachomatis. Journal of Bacteriology 189, 6222-6235.
  • Jun Dai, et al. (2005) The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment. Genes and Development 19, 472-488.
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    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB396VM pET-45b(+) Vector Map