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70225 pBACgus-6 DNA

pBACgus-6 DNA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70225
  
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Áttekintés

Replacement Information
Description
Overview

This product has been discontinued.





pBACgus-6 is a baculovirus transfer plasmid compatible with BacVector™-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection. A modified gp64 tandem promoter contains both an immediate early promoter for expression beginning immediately after infection and a late promoter for continued expression in the late phase of infection. The pBACgus-6 Transfer Plasmid enables isolation of stably-transfected insect cell lines expressing target proteins. A 20 amino acid gp64 signal peptide sequence is capable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. Unique restriction sites are indicated on the circle map. The cloning/expression region of the coding strand transcribed from the gp64 promoter is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.
Catalogue Number70225
Brand Family Novagen®
References
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
70225 0

Documentation

pBACgus-6 DNA MSDS

Title

Safety Data Sheet (SDS) 

pBACgus-6 DNA Certificates of Analysis

TitleLot Number
70225

Brochure

Title
Insect Cell Expression

Citations

Titulus
  • Naoto Yonezawa, et al. (2005) Recombinant porcine zona pellucida glycoproteins expressed in Sf9 cells bind to bovine sperm but not to porcine sperm. Journal of Biological Chemistry 280, 20189-20196.
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    Vector Map

    Title
    TB222 pBACgus-6 Vector Map

    Vector Sequence

    Title
    pBACgus-6 Sequence