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70089 pBACgus-3 DNA

70089
  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Overview

This product has been discontinued.





pBACgus-3 is a baculovirus transfer plasmid compatible with BacVector™-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter and expression of secreted proteins. A 20 amino acid gp64 signal peptide sequence iscapable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. pBACgus-3 provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.
Catalogue Number70089
Brand Family Novagen®
References
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
70089 0

Documentation

pBACgus-3 DNA MSDS

Title

Safety Data Sheet (SDS) 

pBACgus-3 DNA Certificates of Analysis

TitleLot Number
70089

Brochure

Title
Insect Cell Expression

Citations

Titulus
  • Austin N. Kirschner, et al. (2006) Soluble Epstein-Barr virus glycoproteins gH, gL, and gp42 form a 1:1:1 stable complex that acts like soluble gp42 in B-cell fusion but not in epithelial cell fusion. Journal of Virology 80, 9444-9454.
  • Maisoa P. McShane, et al. (2003) Mutational anaylsis of the HLA Class II interaction with Epstein-Barr virus glycoprotein 42. Journal of Virology 77, 7655-7662.
  • Vector Map

    Title
    TB218 pBACgus-3 Vector Map

    Vector Sequence

    Title
    pBACgus-3 Sequence