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03-188 p300 (catalytic domain) human recombinant

Human recombinant p300 (catalytic domain) expressed in E. Coli.

p300 (catalytic domain) human recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
03-188
100 µg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Key ApplicationsSpecies
Enzyme AssaysHuman
Description
Catalogue Number03-188
Brand Family Upstate
Trade Name
  • Upstate
Descriptionp300 (catalytic domain) human recombinant
OverviewHuman recombinant p300 (catalytic domain) expressed in E. Coli
Background InformationAlthough originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, p300 and its paralogue, CBP, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase ß and nuclear import factors. p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. In addition to functions which overlap with those of CBP, p300 has a number of unique functions, including a role in the induction of p21Waf1/Cip1 cell cycle inhibitor.
References
Product Information
FormatPurified
PresentationIn liquid containing 50 mM Tris/HCl, pH 8.0, 0.1 mM EDTA, 10% glycerol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationHuman recombinant p300 (catalytic domain) expressed in E. Coli.
Key Applications
  • Enzyme Assays
Application NotesAcetyltransferase assays
Biological Information
HostE. coli
PurityPurity >75% by SDS-PAGE
SourceCatalytic domain of huma p300
Specific Activity>250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386. CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis (2-nitrobenzoic acid).
Molecular Weight~52 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 12 months at -80ºC from date of receipt. Avoid multiple freeze/thaw cycles.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
03-188 04053252550829

Documentation

p300 (catalytic domain) human recombinant MSDS

Title

Safety Data Sheet (SDS) 

p300 (catalytic domain) human recombinant Certificates of Analysis

TitleLot Number
p300 (catalytic domain) human recombinant - 2315724 2315724
p300 (catalytic domain) human recombinant - 3011124 3011124
p300 (catalytic domain) human recombinant - 3509455 3509455
p300 (catalytic domain) human recombinant - 3739359 3739359
p300 (catalytic domain) human recombinant - 3887982 3887982
p300 (catalytic domain) human recombinant - 4165023 4165023
p300 (catalytic domain) human recombinant - Q2022473 Q2022473
p300 (catalytic domain) human recombinant -2628984 2628984
p300 (catalytic domain) human recombinant Recombinant Protein 3128450
p300 (catalytic domain) human recombinant Recombinant Protein 3143747