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14-352-D cRAF (RAF1)

Recombinant human RAF1, also known as c-RAF, amino acids 306-648, containing the mutations Y340D and Y341D, with N terminal GST tag for use in enzyme kinetics, screening inhibitors, and selectivity profiling.

cRAF (RAF1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-352-D
10 μg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-352M when ordering the 250 µg size.
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue Number14-352-D
Brand Family Upstate
Trade Name
  • Upstate
DescriptioncRAF (RAF1)
OverviewRecombinant human RAF1, also known as c-RAF, amino acids 306-648, containing the mutations Y340D and Y341D, with N terminal GST tag
References
Product Information
Presentation40 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 16 mM glutathione, 20% glycerol, 3 mM DTT
Quality LevelMQ100
Applications
ApplicationRecombinant human RAF1, also known as c-RAF, amino acids 306-648, containing the mutations Y340D and Y341D, with N terminal GST tag for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
Key Applications
  • Kinase Assay
Biological Information
Concentration0.71 mg/ml
Purity≥70%
SourceSf9 insect cells
Specific Activity158 pmol/min/µg
Entrez Gene Number
Entrez Gene SummaryRaf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
Gene Symbol
  • RAF1
  • C-RAF
  • cRaf
  • Raf-1
  • c-Raf
  • CRAF
  • RAF
Target Sub-FamilyTKL
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
Molecular Weight65 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Kinase assay: 10 μM ATP, 1 μM MEK1(K97R), 1 hour.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for > 6 month at –80°C from date of receipt.
Packaging Information
Material Size10 μg
Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-352M when ordering the 250 µg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
14-352-D 04055977276541