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71186 YeastBuster™ Protein Extraction Reagent

71186
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Products

KatalógusszámCsomagolás Menny./csomag
71186-3 Muanyagampulla 100 ml
71186-4 Muanyagampulla 500 ml
Description
OverviewYeastBuster™ Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.

In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind™ and Ni-NTA His•Bind® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.








Catalogue Number71186
Brand Family Novagen®
Features and benefits
  • Gentle, rapid, efficient extraction of proteins from yeast cells
  • Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
  • Higher yield of total and enzymatically active proteins
  • Fully compatible with Ni-NTA His•Bind® and GST•Bind™ affinity purification methods
References
Product Information
Components
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage Multiple storage requirements
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
71186-3 04055977254044
71186-4 04055977254051

Documentation

YeastBuster™ Protein Extraction Reagent MSDS

Title

Safety Data Sheet (SDS) 

YeastBuster™ Protein Extraction Reagent Certificates of Analysis

TitleLot Number
71186

Brochure

Title
Protein Purification and Detection Tools
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression
Western Blotting Tools

Technical Info

Title
TB316 YeastBuster Protein Extraction Reagent

Citations

Titulus
  • Jennifer A. Gibbons, et al. (2007) Expression of human protein phosphatase-1 in Saccharomyces cerevisiae highlights the role of phosphatase isoforms in regulating eukaryotic functions. Journal of Biological Chemistry 282, 21838-21847.
  • Alyssa Dilla, et al. (2004) The Arabidopsis F-Box protein SLEEPY1 targets gibberellin signaling repressors for gibberellin-induced degradation. The Plant Cell 16, 1392-1405.
  • User Protocols

    Title
    TB316 YeastBuster™ Extraction Reagent